Experimental and statistical reevaluation provides no evidence for Drosophila courtship song rhythms.
Experimental and statistical reevaluation provides no evidence for Drosophila courtship song rhythms.
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实验和统计重新评估没有提供果蝇求爱歌曲节奏的证据。
DOI：
10.1073/pnas.1707471114
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发表时间：
2017
影响因子：
11.1
通讯作者：
Murthy,Mala
中科院分区：
文献类型：
--
作者：
Stern,DavidL;Clemens,Jan;Coen,Philip;Calhoun,AdamJ;Hogenesch,JohnB;Arthur,BenJ;Murthy,Mala
从1980年到1992年，一系列有影响力的论文报道了果蝇求偶鸣声脉冲间隔的周期性循环的发现、遗传学和进化。这种周期性背后的分子机制从未被描述过。为了重新开始对这一现象的研究，我们之前对歌曲进行了自动分割，但未能检测到提议的节奏[Arthur BJ， et al. (2013)BMC Biol11:11；李志强，李志强，等。[j].中国生物医学工程学报，2014,12(3):391 - 391。Kyriacou等人[Kyriacou CP， et al. (2017)Proc Natl Acad Sci USA114:1970-1975]报告说，我们未能检测到歌曲节奏，因为(i)我们的苍蝇唱得不够，（ii）我们的分割器没有识别出许多歌曲脉冲。Kyriacou等人手工注释了我们记录的一个子集，并报道了两个菌株显示出具有基因型特异性周期性的节律，与他们的原始报告一致。我们无法复制这一发现，并表明手工注释数据、原始自动分割数据和新数据集都没有提供证据证明基因型之间存在歌曲节奏或歌曲周期性差异。此外，我们重新检查了我们的方法和分析，发现我们的自动分割方法没有偏见，以防止检测假定的歌曲周期性。我们的结论是，没有证据表明果蝇求偶歌曲节奏的存在。
From 1980 to 1992, a series of influential papers reported on the discovery, genetics, and evolution of a periodic cycling of the interval betweenDrosophilamale courtship song pulses. The molecular mechanisms underlying this periodicity were never described. To reinitiate investigation of this phenomenon, we previously performed automated segmentation of songs but failed to detect the proposed rhythm [Arthur BJ, et al. (2013)BMC Biol11:11; Stern DL (2014)BMC Biol12:38]. Kyriacou et al. [Kyriacou CP, et al. (2017)Proc Natl Acad Sci USA114:1970–1975] report that we failed to detect song rhythms because (i) our flies did not sing enough and (ii) our segmenter did not identify many of the song pulses. Kyriacou et al. manually annotated a subset of our recordings and reported that two strains displayed rhythms with genotype-specific periodicity, in agreement with their original reports. We cannot replicate this finding and show that the manually annotated data, the original automatically segmented data, and a new dataset provide no evidence for either the existence of song rhythms or song periodicity differences between genotypes. Furthermore, we have reexamined our methods and analysis and find that our automated segmentation method was not biased to prevent detection of putative song periodicity. We conclude that there is no evidence for the existence ofDrosophilacourtship song rhythms.