Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos
Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human embryos
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DOI：
10.1016/j.fertnstert.2008.09.084
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发表时间：
2010-01-01
影响因子：
6.7
通讯作者：
Oehninger, Sergio
中科院分区：
文献类型：
--
作者：
Riggs, Ryan;Mayer, Jacob;Oehninger, Sergio
目的：探讨低温保存时间对胚胎存活、着床能力及妊娠结局的影响。设计：回顾性研究。单位：学术三级转诊不孕不育中心。患者：体外受精患者和卵母细胞捐赠周期接受者，他们有冷冻保存的胚胎，并在1986年至2007年期间经历了至少一个解冻周期。干预:没有。主要观察指标：解冻后存活率、着床率、临床妊娠率、流产率、活产率。结果：保存时间长度对体外受精或卵母细胞捐赠周期的解冻后存活无显著影响。或者冷冻在原核或卵裂阶段的胚胎。无论是体外受精还是卵母细胞捐赠周期，储存时间对临床妊娠、流产、植入或活产率均无显著影响。Logistic回归分析表明，冷冻时的储存时间或发育阶段不能预测胚胎存活或妊娠结局。只有卵母细胞年龄、存活率和移植胚胎数量是妊娠结局的积极预测因子。结论：冷冻时间对体外受精或卵母细胞捐赠患者的解冻后存活或妊娠结局没有不利影响。[j] .中国农业科学（英文版），2010;33(3):391 - 391。(C)2010年，美国生殖医学学会。)
Objective: To evaluate the impact of cryopreservation storage duration on embryo survival, implantation competence, and pregnancy outcome.Design: Retrospective study.Setting: Academic tertiary-referral infertility center.Patient(s): In vitro fertilization patients and recipients of oocyte donation cycles who had cryopreserved embryos and underwent at least one thaw cycle from 1986 to 2007.Intervention(s): None.Main Outcome Measure(s): Postthaw survival proportion and implantation, clinical pregnancy, miscarriage, and live birth rates.Result(s): Length of storage time did not have a significant effect on postthaw survival for IVF or oocyte donation cycles. or for embryos frozen at the pronuclear or cleavage stages. There was no significant impact of the duration of storage on clinical pregnancy, miscarriage, implantation, or live birth rate, whether from IVF or oocyte donation cycles. Logistic regression analysis demonstrated that the length of storage time or developmental stage at freezing were not predictive of embryo survival or pregnancy outcome. Only oocyte age, survival proportion, and number of transferred embryos were positive predictors of pregnancy outcome.Conclusion(s): Cryostorage duration did not adversely affect postthaw survival or pregnancy outcome in IVF or oocyte donation patients. (Fertil Steril(R) 2010;93:109-15. (C)2010 by American Society for Reproductive Medicine.)