A case controlled study examining the bladder microbiome in women with Overactive Bladder (OAB) and healthy controls
A case controlled study examining the bladder microbiome in women with Overactive Bladder (OAB) and healthy controls
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DOI：
10.1016/j.ejogrb.2017.04.040
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发表时间：
2017-07-01
影响因子：
2.6
通讯作者：
Duckett, Jonathan
中科院分区：
文献类型：
--
作者：
Curtiss, Natasha;Balachandran, Aswini;Duckett, Jonathan
目的：描述无膀胱症状的健康女性的膀胱微生物组特征，并将其与膀胱过度活动综合征（OAB）患者的膀胱微生物组进行比较。研究设计：将63名OAB女性的MSU样本与35名对照者的尿液进行比较。将尿液离心，得到的沉淀物颗粒重新悬浮在上清液中，在好氧条件下镀48 h，厌氧条件下镀7 d。每个形态不同的菌落都进行了纯度镀。裂解细菌菌落，进行聚合酶链反应扩增16s核糖体RNA基因。该DNA被纯化和测序，允许鉴定细菌属。结果：对照组和DAB患者的平均细菌属数均为5.0个（p = 0.99）。尿路致病菌Proteus （P = 0.01）在OAB女性中更为常见。与对照组相比，OAB患者尿液中乳酸菌属的含量较低（p = 0.02）。总体而言，最常见的细菌是葡萄球菌（在59%的样本中生长）、链球菌（51%）、棒状杆菌（37%）和乳酸杆菌（28%）。从尿液样本中共鉴定出95个不同的属。结论：女性膀胱微生物群具有多样性，OAB患者与对照组的细菌种类差异有统计学意义。(C) 2017 Elsevier B.V.版权所有
Objective: To characterise the microbiome in healthy women with no bladder symptoms and to compare this to the bladder microbiome in patients with overactive bladder syndrome (OAB).Study design: MSU specimens from 63 women with OAB were compared to urine from 35 controls. Urine was centrifuged and the resulting sediment pellet was re-suspended in supernatant and plated under aerobic conditions for 48 h and anaerobic conditions for 7 days. Each morphologically distinct colony was purity plated. Bacterial colonies were lysed and polymerase chain reaction undertaken to amplify the 16 s ribosomal RNA gene. This DNA was purified and sequenced allowing identification of bacterial genera.Results: The mean number of different bacterial genera was 5.0 in both controls and DAB patients (p = 0.99). The uropathogenic bacteria Proteus (P = 0.01) was more commonly isolated from women with OAB. The genus lactobacillus was present less commonly in urine from OAB patients when compared to urine taken from controls (p = 0.02). Overall the most commonly grown bacteria were staphylococcus (grown in 59% of samples), streptococccus (51%), corynebacterium (37%) and lactobacillus (28%). A total of 95 different genera were identified from the urine samples.Conclusion: The female human bladder has a diverse microbiome with stastistically significant differences between bacterial species present in OAB patients and controls. (C) 2017 Elsevier B.V. All rights reserved.