High frequency embryogenesis through isolated microspore culture in Brassica napus L. and B. Carinata braun
High frequency embryogenesis through isolated microspore culture in Brassica napus L. and B. Carinata braun
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DOI：
10.1016/0168-9452(85)90178-5
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发表时间：
1985-07
期刊：
影响因子：
5.2
通讯作者：
P. Chuong;W. Beversdorf
中科院分区：
文献类型：
--
作者：
P. Chuong;W. Beversdorf
对6个甘蓝型油菜品种和1个甘蓝型油菜品种的游离小孢子进行了研究。隆突藻在改良Nitsch和Nitsch（NN）培养基中附加13%（W/V）蔗糖、0.05mg/l苄基腺嘌呤（BA）和1.00mg/l萘乙酸（NAA）。在22至32°C的培养温度下观察到胚胎发生反应。对于大多数测试的基因型，胚的最高频率发生在30°C下，每个花药7-54个胚（约100个胚）。17000个小孢子/花药）发育。虽然在30°C下孵育产生最高频率的胚胎，但较低的培养温度诱导出更好质量的胚胎。在32°C下孵育3天，然后在25°C下孵育的分温培养方案导致高胚胎产量和高百分比的正常胚胎。从小孢子胚发育成植株似乎受到基因型和培养基的影响。
Isolated microspores from six cultivars ofBrassica napusand one ofB. carinatawere cultured in modified Nitsch and Nitsch (NN) medium supplemented with 13% (W/V) sucrose, 0.05 mg/l benzyladenine (BA) and 1.00 mg/l nahpthaleneacetic acid (NAA). Embryogenic responses were observed at cultured temperatures ranging from 22 to 32°C. For most genotypes tested, the highest frequency of embryos occurred at 30°C and 7–54 embryos per anther (approx. 17 000 microspores per anther) developed. Although incubation at 30°C produced the highest frequency of embryos, lower culture temperatures induced better quality embryos. A split temperature culture regime of incubation at 32°C for 3 days followed by incubation at 25°C resulted in both high embryo yields and a high percentage of normal embryos. Plantlet development from microspore-derived embryos appeared to be influenced by both genotype and medium.