Metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production
Metabolic engineering of long chain-polyunsaturated fatty acid biosynthetic pathway in oleaginous fungus for dihomo-gamma linolenic acid production
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DOI：
10.1016/j.jbiotec.2015.12.003
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发表时间：
2016-01-20
影响因子：
4.1
通讯作者：
Laoteng, Kobkul
中科院分区：
文献类型：
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作者：
Chutrakul, Chanikul;Jeennor, Sukanya;Laoteng, Kobkul
微生物油脂是食品、饲料、保健品和医药等领域的长链多不饱和脂肪酸（LC-PUFA）的重要替代来源。二高-γ-亚麻酸（C20：3 Delta（8，11，14）; DGLA）是一种重要的LC-PUFA，具有抗炎和抗增殖作用。为了产生产生DGLA的菌株，通过共表达密码子优化的腐霉Δ（6）-去饱和酶和Δ（6）-延伸酶的代谢工程化在黑曲霉中进行脂肪酸重建，与天然酶相比，其具有高的底物转化为相应产物的转化率。δ（6）-去饱和和δ（6）-延长产物γ-亚麻酸（C18：3 δ（6，9，12）; GLA）和DGLA在磷脂中而不是在三酰甘油中积累。有趣的是，在产油真菌中的脂质质量的操纵并不影响生长和脂质表型。这种策略可能会扩展到开发产油真菌菌株，用于生产其他具有工业应用的定制油。(C)2015爱思唯尔B. V.保留所有权利。
Microbial lipids are promising alternative sources of long chain-polyunsaturated fatty acids (LC-PUFAs) for food, feed, nutraceutical and pharmaceutical sectors. Dihomo-gamma-linolenic acid (C20:3 Delta(8,11,14); DGLA) is an important LC-PUFAs with anti-inflammatory and anti-proliferative effects. To generate a DGLA-producing strain, fatty acid reconstitution in Aspergillus oryzae was performed by metabolic engineering through co-expression of codon-optimized Pythium Delta(6)-desaturase and Delta(6)-elongase, which had high conversion rates of substrates to respective products as compared to the native enzymes. The Delta(6)-desaturated and Delta(6)-elongated products, gamma-linolenic acid (C18:3 Delta(6,9,12); GLA) and DGLA, were accumulated in phospholipids rather than triacylglycerol. Interestingly, the manipulation of lipid quality in the oleaginous fungus did not affect growth and lipid phenotypes. This strategy might expand to development of the oleaginous fungal strain for producing other tailor-made oils with industrial applications. (C) 2015 Elsevier B.V. All rights reserved.