Conservation of antigenic determinants in leucine dehydrogenase from thermophilic and mesophilic Bacillus strains
Conservation of antigenic determinants in leucine dehydrogenase from thermophilic and mesophilic Bacillus strains
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嗜热和嗜温芽孢杆菌菌株亮氨酸脱氢酶中抗原决定簇的保守性
DOI：
10.1111/j.1574-6968.1986.tb01820.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
H. Schütte
中科院分区：
文献类型：
--
作者：
U. Kärst;H. Tsai;H. Schütte
针对从嗜温蜡状芽孢杆菌纯化的八聚体L-亮氨酸脱氢酶（LeuDH）的抗体已被用于筛选LeuDH的16个嗜热芽孢杆菌菌株。其中4株，球形芽孢杆菌461和芽孢杆菌405、406和411，当用Ouchterlony双扩散试验检测时，显示出部分同一性类型的特别强的交叉反应，从而表明它们与B在免疫学上相关。蜡状酶来自嗜热菌株的LeuDH在升高的温度下是非常稳定和高度活性的，并且在Arrhenius图中在约55°C下给出向下的弯曲。L-亮氨酸脱氨基的最佳pH值为pH 11左右的所有菌株检查。
Antibodies against the purified octameric l-leucine dehydrogenase (LeuDH) from the mesophilic Bacillus cereus have been used to screen 16 thermophilic Bacillus strains for LeuDH. 4 of these strains, Bacillus sphaericus 461 and Bacillus sp. 405, 406, and 411, showed a particularly strong cross reaction of the partial identity type when examined by Ouchterlony double diffusion assay, thus indicating that they were immunologically related to the B. cereus enzyme. The LeuDH from the thermophilic strains were very stable and highly active at elevated temperatures, and gave a downward bend at about 55°C in the Arrhenius plot. The pH optimum for l-leucine deamination was around pH 11 for all strains examined.