Effect of propolis on preserving human periodontal ligament cells and regulating pro-inflammatory cytokines
Effect of propolis on preserving human periodontal ligament cells and regulating pro-inflammatory cytokines
复制标题
蜂胶对保护人牙周膜细胞及调节促炎细胞因子的作用
DOI：
10.1111/edt.12411
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发表时间：
2018-08-01
影响因子：
2.5
通讯作者：
Zhao, Yuming
中科院分区：
文献类型：
--
作者：
Yuan, Xiaojing;Wang, Yuanyuan;Zhao, Yuming
背景/目的蜂胶已被建议作为撕脱牙齿的存储介质。本研究的目的是比较巴西蜂胶与汉克平衡盐溶液和牛奶在维持人牙周膜细胞活力、成骨分化潜力和促炎细胞因子表达方面的有效性。材料和方法采用细胞计数试剂盒 8 检测来测试不同储存介质中人牙周膜细胞的活力。使用碱性磷酸酶染色和活性测定、茜素红 S 染色和蛋白质印迹评估对成骨分化的保存作用。使用实时 PCR 和酶联免疫吸附测定法对促炎细胞因子进行定量。结果 10g/mL 的巴西蜂胶对人牙周膜细胞没有细胞毒性。牛奶组显示出最高的细胞活力。巴西蜂胶和汉克平衡盐溶液组显示出相似的细胞活力。所有组中的碱性磷酸酶染色和活性相似。巴西蜂胶组和汉克平衡盐溶液组的钙沉积和矿化结节形成相似，但牛奶组较高。所有组中的成骨标记基因和蛋白质水平相似。巴西蜂胶处理后IL1、IL6和IL8的基因和蛋白表达水平显着下降。实验组间TNF mRNA表达无显着差异。牛奶组促炎细胞因子水平高于巴西蜂胶组和汉克平衡盐溶液组。结论巴西蜂胶、汉克平衡盐溶液和牛奶组维持了人牙周膜细胞的活力，并同样保留了其成骨分化能力。然而，巴西蜂胶表现出更好的抗炎作用。
Background/AimPropolis has been suggested as a storage medium for avulsed teeth. The aim of this study was to compare the effectiveness of Brazilian propolis with Hank's balanced salt solution and milk in maintaining the viability of human periodontal ligament cells, their osteogenic differentiation potential, and pro-inflammatory cytokine expression.Material and MethodsCell Counting Kit 8 assays were performed to test human periodontal ligament cell viability in different storage media. The preservative effect on osteogenic differentiation was evaluated using alkaline phosphatase staining and activity assays, Alizarin Red S staining, and western blotting. Quantification of pro-inflammatory cytokines was performed using real-time PCR and enzyme-linked immunosorbent assays.ResultsBrazilian propolis at 10g/mL was not cytotoxic toward human periodontal ligament cells. The milk group showed the highest cell viability. Brazilian propolis and Hank's balanced salt solution groups showed similar cell viabilities. Alkaline phosphatase staining and activity were similar in all groups. Calcium deposition and mineralization nodule formation were similar in the Brazilian propolis and Hank's balanced salt solution groups but were higher in the milk group. Osteogenic marker gene and protein levels were similar in all groups. The genes and protein expression levels of IL1, IL6, and IL8 decreased significantly after treatment with Brazilian propolis. TNF mRNA expression showed no significant difference among the experimental groups. Pro-inflammatory cytokine levels in the milk group were higher than in the Brazilian propolis and Hank's balanced salt solution groups.ConclusionsBrazilian propolis, Hank's balanced salt solution, and milk maintained the viability of human periodontal ligament cells and preserved their osteogenic differentiation ability similarly. However, Brazilian propolis showed a better anti-inflammatory effect.