The effects of neonatal castration on the subsequent behavioural response to centrally administered arginine vasopressin and the expression of V1a receptors in adult male prairie voles
The effects of neonatal castration on the subsequent behavioural response to centrally administered arginine vasopressin and the expression of V1a receptors in adult male prairie voles
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DOI：
10.1046/j.1365-2826.2003.01097.x
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发表时间：
2003-11-01
影响因子：
3.2
通讯作者：
Young, LJ
中科院分区：
文献类型：
--
作者：
Cushing, BS;Okorie, U;Young, LJ
中枢注射精氨酸加压素诱导雄性草原田鼠(Microtus Ochrogaster)形成伴侣偏好。许多加压素调节的行为的表达依赖于睾酮。在这项研究中，我们测试了这样一种假设，即早期暴露于性腺类固醇对于建立成年雄性草原田鼠对外源加压素的典型反应是必要的，并预测成年雄性田鼠在新生儿时期被去势后，不会因中央注射加压素而形成伴侣偏好。我们还检测了新生儿去势对血管加压素(V-1a)受体表达的影响。田鼠在出生当天被阉割(NEOCAST)，出生当天被假阉割(NEOSHAM)或成年后被阉割(ADULTCAST)。除了作为加压素作用对照的一组假雄性新生儿(NEOSHAM CON)外，所有成年男性都接受了1-MUL脑室内人工脑脊液中注射加压素(100 Ng)。此外，在测试前2周，一组新生儿去势男性接受了丙酸睾丸酮植入(NEOCAST+TP)。在60日龄至90日龄期间，在侧脑室放置内插管，24小时后，雄性动物注射加压素。随后，在额外的15分钟后，雄性动物与雌性“伴侣”同居1小时。同居后，雄性动物立即被放入Y型伴侣偏好测试仪中3小时，在该仪器中，雄性动物可以接触到“伴侣”，而雌性动物则可以接触到“陌生人”。在治疗过程中和陌生人在一起的时间被比较了。研究结果支持我们的假设，因为NEOSHAM和ADULTCAST男性形成了伴侣偏好，与伴侣相处的时间更多，而且他们与伴侣在一起的时间明显多于NEOSHAM CON、NEOCAST或NEOCAST+TP男性。在新生儿去势的男性中，替代睾酮并不能恢复成年男性对加压素的反应，从而恢复伴侣偏好的形成。最后，新生儿去势不影响V-1a受体的分布。
Centrally administered arginine vasopressin induces the formation of partner preferences in male prairie voles (Microtus ochrogaster). The expression of many vasopressin-regulated behaviours is testosterone dependent. In this study, we tested the hypothesis that early exposure to gonadal steroids are necessary to establish the typical response of adult male prairie voles to exogenous vasopressin, predicting that adult males which were castrated neonatally would not form partner preferences in response to centrally administered vasopressin. We also examined the effect of neonatal castration on the expression of vasopressin (V-1a) receptors. Voles were castrated on the day of birth (NEOCAST), sham-castrated on the day of birth (NEOSHAM) or castrated as adults (ADULTCAST). With the exception of one group of neonatal sham males (NEOSHAM CON), which served as a control for the effects of vasopressin, as adults, all males received a 1-mul intracerebroventricular injection of vasopressin (100 ng) in artificial cerebrospinal fluid. In addition, 2 weeks before testing, one group of neonatally castrated males received an implant of testosterone propionate (NEOCAST + TP). Between 60 and 90 days of age, an internal cannula was placed in the lateral cerebral ventricle and, 24 h later, males were injected with vasopressin. Subsequently, after an additional 15 min, males were cohabitated with a female 'partner' for 1 h. Immediately following cohabitation, males were placed in a Y-shaped partner preference test apparatus for 3 h, in which the male had access to the 'partner' and a novel female, 'stranger.' Time spent with the partner versus the stranger was compared within and between treatments. The results were found to support our hypothesis as the NEOSHAM and ADULTCAST males formed partner preferences, spending more time with the partner, and they spent significantly more time with their partner than did NEOSHAM CON, NEOCAST or NEOCAST + TP males. Replacement of testosterone in neonatally castrated males did not restore partner preference formation in response to vasopressin in adult males. Finally, neonatal castration did not affect the distribution of V-1a receptors.