Ovarian transforming growth factor-alpha gene expression: immunohistochemical localization to the theca-interstitial cells.
Ovarian transforming growth factor-alpha gene expression: immunohistochemical localization to the theca-interstitial cells.
复制标题
卵巢转化生长因子-α 基因表达：免疫组织化学定位于卵泡膜间质细胞。
作者：
J. Kudlow;M. Kobrin;A. Purchio;D. Twardzik;E. Hernández;S. Asa;E. Adashi
检测大鼠卵巢中是否存在转化生长因子-α(TGF-α)。未成熟的己烯雌酚诱导的大鼠在处死前用FSH或不用FSH治疗3天。用甲醛固定卵巢进行免疫组织化学染色，或提取RNA进行Northern印迹分析。与大鼠转化生长因子-αc DNA探针杂交显示，FSH处理的大鼠其丰度显著增加，为4.5kb的m RNA。用抗转化生长因子-α的序列特异性单抗MF9进行免疫组织化学染色，仅在间质和膜细胞中检测到转化生长因子-α。FSH处理后免疫染色无明显变化。这些结果表明，转化生长因子-α是在卵巢合成的，可能是在膜间质中合成的。
Rat ovaries were examined for the presence of transforming growth factor-alpha (TGF-alpha). Immature diethylstilbestrol-primed rats were treated for 3 days prior to sacrifice with or without FSH. Their ovaries were either fixed in formaldehyde for immunohistochemistry or the RNA was extracted for northern blot analysis. Hybridization with a rat TGF-alpha cDNA probe revealed a 4.5 kilobase mRNA whose abundance was markedly stimulated in the rats treated with FSH. Immunohistochemical staining, using a sequence specific monoclonal antibody to TGF-alpha, MF9, detected immunoreactive TGF-alpha only in the interstitial and theca cells. FSH treatment resulted in no appreciable change in the immunostaining. These results suggest that TGF-alpha is synthesized in the ovary, perhaps in the theca-interstitial compartment.