Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae
Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae
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DOI：
10.1111/j.1574-6968.2006.00395.x
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发表时间：
2006-09-01
影响因子：
2.1
通讯作者：
Fujiwara, Toru
中科院分区：
文献类型：
--
作者：
Nozawa, Akira;Takano, Junpei;Fujiwara, Toru
研究了BOR 1、DUR 3和FPS 1三种膜蛋白在酵母硼（B）转运中的作用。缺乏BOR1的酵母细胞中的硼浓度在暴露于90 mM硼酸后升高，而缺乏DUR3或FPS1的细胞显示出较低的硼浓度。与对照细胞相比，过表达BOR1或FPS1的细胞具有较低的硼浓度，而过表达DUR3的细胞具有较高的硼浓度。这些结果表明，除了外排硼转运蛋白BOR1，DUR3和FPS1在调节细胞硼浓度中发挥重要作用。酵母转化体的耐高硼酸浓度的分析表明，原生质硼浓度和耐高外部硼浓度的程度之间存在明显的负相关性。因此，BOR1，DUR3，和FPS1似乎参与耐受硼酸和维持原生质硼浓度。
The roles of three membrane proteins, BOR1, DUR3, and FPS1, in boron (B) transport in yeast were examined. The boron concentration in yeast cells lacking BOR1 was elevated upon exposure to 90 mM boric acid, whereas cells lacking DUR3 or FPS1 showed lower boron concentrations. Compared with control cells, cells overexpressing BOR1 or FPS1 had a lower boron concentration, and cells overexpressing DUR3 had a higher boron concentration. These results suggest that, in addition to the efflux boron transporter BOR1, DUR3 and FPS1 play important roles in regulating the cellular boron concentration. Analysis of the yeast transformants for tolerance to a high boric acid concentration revealed an apparent negative correlation between the protoplasmic boron concentration and the degree of tolerance to a high external boron concentration. Thus, BOR1, DUR3, and FPS1 appear to be involved in tolerance to boric acid and the maintenance of the protoplasmic boron concentration.