Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans
Genomic mutation rates for lifetime reproductive output and lifespan in Caenorhabditis elegans
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DOI：
10.1073/pnas.94.8.3823
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发表时间：
1997-04-15
影响因子：
11.1
通讯作者：
Caballero, A
中科院分区：
文献类型：
--
作者：
Keightley, PD;Caballero, A
有关性别进化、重组和突变负荷的理论依赖于有害突变影响的速率和分布信息。关于果蝇基因组突变率的直接信息表明，轻度有害突变的积累使种群的生存能力每代至少降低1%。我们进行了一项实验来测量秀丽隐杆线虫的有害突变率，其中每一代由一个雌雄同体亲本维持独立的亚系，这种条件最大限度地减少了自然选择的机会，并导致有害突变的随机固定。经过60代的突变积累，平均生殖产量和寿命的变化可以忽略不计，但遗传变异以其他物种生活史性状的典型速率增加。估计每个单倍体基因组的适应度有害突变率为0.0026，比之前在果蝇中测量的生存能力低两个数量级。
Theory concerning the evolution of sex and recombination and mutation load relies on information on rates and distributions of effects of deleterious mutations. Direct information on the genomic mutation rate in Drosophila implies that an accumulation of mildly deleterious mutations reduces viability of populations by at least 1% per generation. We carried out an experiment to measure the deleterious mutation rate in Caenorhabditis elegans, in which independent sublines were maintained with one hermaphrodite parent per generation, conditions that minimize the opportunity for natural selection and lead to random fixation of deleterious mutations. After 60 generations of mutation accumulation, negligible changes in mean reproductive output and lifespan occurred, but the genetic variance increased at rates typical for life history traits in other species. The estimated deleterious mutation rate per haploid genome for fitness, U, was 0.0026, a figure two orders of magnitude smaller than previously measured for viability in Drosophila.