Evidence that a cytoplasmically located version of a v-erbB-encoded protein can transform both fibroblasts and erythroblasts.
Evidence that a cytoplasmically located version of a v-erbB-encoded protein can transform both fibroblasts and erythroblasts.
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有证据表明，位于细胞质的 v-erbB 编码蛋白可以转化成纤维细胞和成红细胞。
DOI：
10.1016/0042-6822(92)91253-q
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
Hayman,MJ
中科院分区：
文献类型：
--
作者：
Lee,EB;Meyer,S;Hayman,MJ
我们先前分离了一种禽红母细胞病病毒AEV-GEE35，其中v- erbboncoprotein的完整胞外和跨膜结构域被gag和env蛋白的序列所取代。GEE35病毒能够像野生型v-erbB一样有效地转化成纤维细胞和红母细胞。对GEE35编码的v- erbb蛋白进行分析，发现两种分子量相近的蛋白约为130,000 Da。其中一种是n -连接糖基化膜蛋白，而另一种是细胞质蛋白。这两种蛋白的生化表征表明，跨膜蛋白在细胞外有它们的v- erbb结构域，因此它不再能够接触到酪氨酸激酶底物。这表明是细胞质上定位的v- erbb编码蛋白负责这种病毒的有效转化能力。
We previously isolated an avian erythroblastosis virus, AEV-GEE35, in which the complete extracellular and transmembrane domains of thev-erbBoncoprotein were replaced with sequences from the gag and env proteins. The GEE35 virus was capable of transforming both fibroblasts and erythroblasts as efficiently as wild-type v-erbB. Analysis of thev-erbBproteins encoded by GEE35 revealed two proteins of similar molecular weights of approximately 130,000 Da. One of these proteins was an N-linked glycosylated membrane protein, whereas the other was a cytoplasmic protein. Biochemical characterization of these two proteins revealed that the transmembrane protein has thev-erbBdomain outside the cell, such that it no longer had access to its tyrosine kinase substrates. This implies that it is the cytoplasmically locatedv-erbB-encoded protein that is responsible for the efficient transforming ability of this virus.