Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs
复制标题
Omentin-1 改善游离脂肪酸引起的 HUVEC 增殖、迁移和炎症状态损伤
DOI：
10.1155/2020/3054379
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发表时间：
2020-03-27
影响因子：
2.1
通讯作者：
Luo, Fanyan
中科院分区：
文献类型：
--
作者：
Chen, Yubin;Liu, Fen;Luo, Fanyan
目的内皮细胞损伤是动脉粥样硬化发展过程中的重要病理变化。在此，我们探讨网膜素-1对游离脂肪酸（FFA-）诱导的内皮细胞损伤的影响。方法建立FFA诱导的内皮细胞损伤模型，观察网膜素-1在此过程中的作用。用细胞计数试剂盒测定和流式细胞术分析细胞增殖。划痕和transwell试验用于评估细胞迁移。采用免疫印迹法、逆转录定量聚合酶链反应和细胞荧光法检测内皮细胞损伤后分泌的相关因子。结果Omentin-1可改善FFA诱导的人脐静脉内皮细胞（HUVECs）增殖和迁移能力的损伤。它减少了THP-1细胞的数量连接到HUVECs在响应损伤和抑制FFA诱导的促炎状态的HUVECs。结论Omentin-1可部分减轻FFA诱导的内皮细胞损伤。
Objectives Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury. Methods An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay. Results Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs. Conclusion Omentin-1 could partly ameliorate FFA-induced endothelial cell injury.