Mycoplasma hominis and Gardnerella vaginalis display a significant synergistic relationship in bacterial vaginosis
Mycoplasma hominis and Gardnerella vaginalis display a significant synergistic relationship in bacterial vaginosis
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DOI：
10.1007/s10096-015-2564-x
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发表时间：
2016-03-01
影响因子：
4.5
通讯作者：
Coyle, P. V.
中科院分区：
文献类型：
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作者：
Cox, C.;Watt, A. P.;Coyle, P. V.
阴道加德纳菌在细菌性阴道病（BV）中起重要作用，而生殖器毛菌的作用不太明显。使用目前的革兰氏染色纽金特评分来诊断细菌性阴道炎，对于确定缺乏细胞壁的分子的作用也是次优的。由于细菌负荷和多样性是BV的重要先决条件，实时定量聚合酶链反应（qPCR）检测可以对这些进行评估。本研究的目的是确定生殖器Mollicutes的作用和潜在的模式协同阴道阴道杆菌在女性细菌性阴道炎。用Nugent评分将130名女性阴道拭子分类为BV （n = 28）、中度（n = 22）和非BV (n = 80)，对其进行了四种qPCR TaqMan检测，检测目标为阴道支原体、人支原体、生殖器支原体、解脲支原体和细小支原体。统计分析用于比较三组妇女的细菌患病率和负荷。人原体和阴道支原体合并感染在细菌性阴道炎患者中（60.7%）明显高于中度（36.4%）和非细菌性阴道炎患者（8.8%）（p < 0.001）。BV女性人支原体和阴道支原体感染负荷显著增高（p = 0.001），人支原体和阴道支原体感染负荷显著正相关（p < 0.001; r - 0.60）。与其他莫里特人没有明显的联系。这些发现加强了人支原体在细菌性阴道炎中的作用以及与阴道支原体的潜在协同作用的证据。这种协同作用可能是这种疾病和性接触的重要触发因素，性接触是一种共生细菌传播的渠道，而共生细菌可能会引发这种疾病。
Gardnerella vaginalis plays an important role in bacterial vaginosis (BV,) while the role of genital Mollicutes is less obvious. The diagnosis of BV by use of the current Gram stain Nugent score is also suboptimal for defining the role of Mollicutes that lack a cell wall. Since bacterial load and diversity is an important prerequisite for BV, real-time quantitative polymerase chain reaction (qPCR) assays enable these to be assessed. The purpose of this study was to define the role of genital Mollicutes and potential patterns of synergy with G. vaginalis in women with BV. Vaginal swabs from 130 women categorised by Nugent score as BV (n = 28), intermediate (n = 22) and non-BV (n = 80) were tested against four qPCR TaqMan assays targeting G. vaginalis, Mycoplasma hominis, M. genitalium, Ureaplasma urealyticum and U. parvum. Statistical analyses were used to compare bacterial prevalence and load between the three groups of women. Mycoplasma hominis and G. vaginalis co-infection was significantly more common in BV (60.7 %) compared to intermediate (36.4 %) and non-BV (8.8 %) Nugent scores (p < 0.001). Significantly higher loads of M. hominis (p = 0.001) and G. vaginalis (p < 0.001) were detected in women with BV and the respective loads in M. hominis and G. vaginalis co-infections displayed a significant positive correlation (p < 0.001; r - 0.60). No significant associations were seen with the other Mollicutes. The findings strengthen the evidence of a role for M. hominis in BV and a potential synergy with G. vaginalis. This synergy could be an important trigger of the condition and sexual contact the conduit for the transmission of an otherwise commensal bacterium that could initiate it.