Multiple colonization defects in a cysteine protease mutant of Porphyromonas gingivalis
Multiple colonization defects in a cysteine protease mutant of Porphyromonas gingivalis
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DOI：
10.1111/j.1600-0765.1997.tb01395.x
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Cho, MI
中科院分区：
文献类型：
--
作者：
Kuramitsu, H;Tokuda, M;Cho, MI
最近在本实验室构建了一株rqp-1基因缺陷的牙龈卟啉单胞菌381的半胱氨酸蛋白酶突变体G-102。为了评价该酶在牙龈假单胞菌毒力特性中的作用，我们对该突变体的一些可能的牙周病变特性进行了评估。与亲本菌株相比，突变体G-102在与革兰氏阳性菌和培养的上皮细胞相互作用方面存在缺陷。此外，突变体在附着细胞外基质的蛋白质组分以及I型胶原方面也发生了变化。其中一些变化可能是由于突变体G-102相对于菌株381表现出的自聚集性降低所致。然而，由于上皮细胞附着试验是在非常低的细菌密度下进行的，因此突变体的自我聚集减少不太可能是其附着到这些真核细胞的能力降低的原因。细胞的电子显微镜检查还显示，突变的G-102在正常的菌毛表达中发生了变化。此外，Western和Northern杂交检测到突变体中43 kDa菌毛亚基的表达降低。这些结果表明，RGP-1基因产物可以直接或间接地影响牙龈假单胞菌的定植特性。
A cysteine protease mutant, G-102, of Porphyromonas gingivalis 381 defective in the rqp-1 gene has been recently constructed in this laboratory. In order to evaluate the role of the protease in the virulence properties of P. gingivalis, a number of putative periodontopathic properties of the mutant were evaluated. Relative to the parental strain, mutant G-102 was demonstrated to be defective in interacting with Gram-positive bacteria as well as cultured epithelial cells. In addition, the mutant was altered in attaching to the protein components of extracellular matrix as well as to type I collagen. Some of these alterations could result from the decreased autoaggregation displayed by mutant G-102 relative to strain 381. However, since the epithelial cell attachment assays were carried out at very low bacterial densities, it is unlikely that reduced autoaggregation of the mutant is responsible for its decreased ability to attach to these eucaryotic cells. Electron microscopic examination of The cells also revealed that mutant G-102 was altered in normal fimbrae expression. In addition, reduced expression of the 43 kDa fimbrial subunit in the mutant was detected with both Western and Northern blotting. These results indicated that the rgp-1 gene product can play either a direct or indirect role in the colonization properties of P. gingivalis.