A precise method for replicating suspension cultures of mammalian cells.
A precise method for replicating suspension cultures of mammalian cells.
复制标题
复制哺乳动物细胞悬浮培养物的精确方法。
DOI：
10.1128/aem.16.6.841-844.1968
复制
发表时间：
1968
期刊：
影响因子：
--
通讯作者：
R. E. Lincoln
中科院分区：
文献类型：
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作者：
F. J. Weirether;J. Walker;R. E. Lincoln
描述了一种用于培养哺乳动物细胞的简单、容易组装的摇瓶培养系统。在该系统中没有使用专门的玻璃器皿和设备，该系统由配备呼吸器取样组件的锥形瓶组成。该单位用于定量几个变量的影响，包括培养基成分、连续转移以及冷冻和储存对L细胞系的两种变体的影响。该系统是可再现的和精确的，并且允许细胞在悬浮液中生长延长的时间段。大量的细胞可以大量生产。可以同时运行多个重复，以产生用于统计分析的数据。
A simple, readily assembled shaker-culture system for the cultivation of mammalian cells is described. No specialized glassware and equipment were used in this system, which consists of an Erlenmeyer flask fitted with a breather-sampling assembly. This unit was employed to quantitate the effects of several variables, including medium ingredients, serial transfers, and freezing and storage on two variants of the L-cell line. This system is reproducible and precise and allows for growth of cells in suspension for extended periods of time. Large numbers of cells can be mass-produced. Many replicates can be run simultaneously to yield data for statistical analysis.