Otx/otd homeobox genes specify distinct sensory neuron identities in C-elegans
Otx/otd homeobox genes specify distinct sensory neuron identities in C-elegans
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DOI：
10.1016/s1534-5807(03)00293-4
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发表时间：
2003-10-01
影响因子：
11.8
通讯作者：
Sengupta, P
中科院分区：
文献类型：
--
作者：
Lanjuin, A;VanHoven, MK;Sengupta, P
神经元的不同功能特性产生的机制知之甚少。C.秀丽线虫使用12种类型的感觉神经元对热和化学刺激作出反应。Otx/otd同源物ttK-1指定AFD热敏神经元的身份。我们在这里显示，ceh-36和ceh-37，在C. elegans基因组，指定AWC，ASE和AWB化学感觉神经元的身份，定义了这个基因家族在感觉神经元特化中的作用。全是C。线虫OtK基因和大鼠Otx 1可以替代ceh-37和ceh-36，但只有ceh-37在功能上替代ttx-1。AWB神经元中的功能取代是由不同的Otx基因激活相同的下游靶点lim-4介导的。错误表达实验表明，虽然特定的身份通过后，OTx基因的表达可能会受到细胞环境的限制，个别OTx基因优先促进不同的神经元的身份。
The mechanisms by which the diverse functional identities of neurons are generated are poorly understood. C. elegans responds to thermal and chemical stimuli using 12 types of sensory neurons. The Otx/otd homolog ttK-1 specifies the identities of the AFD thermosensory neurons. We show here that ceh-36 and ceh-37, the remaining two Otx-like genes in the C. elegans genome, specify the identities of AWC, ASE, and AWB chemosensory neurons, defining a role for this gene family in sensory neuron specification. All C. elegans OtK genes and rat Otx1 can substitute for ceh-37 and ceh-36, but only ceh-37 functionally substitutes for ttx-1. Functional substitution in the AWB neurons is mediated by activation of the same downstream target lim-4 by different Otx genes. Misexpression experiments indicate that although the specific identity adopted upon expression of an Otx gene may be constrained by the cellular context, individual Otx genes preferentially promote distinct neuronal identities.