Cdx1 and Cdx2 expression during intestinal development
Cdx1 and Cdx2 expression during intestinal development
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DOI：
10.1053/gast.2000.18142
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发表时间：
2000-10-01
期刊：
影响因子：
29.4
通讯作者：
Traber, PG
中科院分区：
文献类型：
--
作者：
Silberg, DG;Swain, GP;Traber, PG
背景与目的：肠道特异性转录因子Cdx1和Cdx2是指导肠道发育、分化和维持肠道表型的候选基因。本研究的重点是小鼠胃肠道发育过程中Cdx1和Cdx2的复杂表达模式。方法：采用免疫组化方法检测小鼠胚胎和出生后组织中Cdx1和Cdx2蛋白的表达。结果：Cdx2蛋白在交配后9.5 （pc）出现表达，而Cdx1蛋白在发育中的远端肠（后肠）12.5 （pc）首次出现弱表达。Cdx1的表达在内胚层/上皮过渡期间从13.5增加到14.5，并以远端表达为主。与Cdx1相反，Cdx2在除远端部分外的所有发育中的肠中都有强烈表达。在整个出生后发育过程中，Cdx2在远端结肠的表达一直很低。在隐窝-绒毛轴上形成表达梯度，Cdx1主要在隐窝中表达，Cdx2主要在绒毛中表达。结论：本研究对Cdx1和Cdx2蛋白在发育过程中的表达模式进行了直接比较，为其潜在的功能作用提供了新的见解。Cdx1和Cdx2蛋白的相对表达可能在肠上皮的前后模式以及沿隐窝绒毛轴的增殖和分化模式中起重要作用。
Background & Aims: The intestine-specific transcription factors Cdx1 and Cdx2 are candidate genes for directing intestinal development, differentiation, and maintenance of the intestinal phenotype. This study focused on the complex patterns of expression of Cdx1 and Cdx2 during mouse gastrointestinal development. Methods: Embryonic and postnatal mouse tissues were analyzed by immunohistochemistry to determine protein expression of Cdx1 and Cdx2 in the developing intestinal tract. Results: Cdx2 protein expression was observed at 9.5 postcoitum (pc), whereas weak expression of Cdx1 protein was first seen at 12.5 pc in the distal developing intestine (hindgut). Expression of Cdx1 increased from 13.5 to 14.5 pc during the endoderm/epithelial transition with predominately distal expression. In contrast to Cdx1, there was intense expression of Cdx2 in all but the distal portions of the developing intestine. Cdx2 expression remained low in the distal colon throughout postnatal development. A gradient of expression formed in the crypt-villus axis, with Cdx1 primarily in the crypt and Cdx2 primarily in the villus. Conclusions: Direct comparison of the patterns of Cdx1 and Cdx2 protein expression during development as performed in this study provides new insights into their potential functional roles. The relative expression of Cdx1 to Cdx2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis.