Age-related losses of cognitive function and motor skills in mice are associated with oxidative protein damage in the brain
Age-related losses of cognitive function and motor skills in mice are associated with oxidative protein damage in the brain
复制标题
DOI：
10.1073/pnas.93.10.4765
复制
发表时间：
1996-05-14
影响因子：
11.1
通讯作者：
Sohal, RS
中科院分区：
文献类型：
--
作者：
Forster, MJ;Dubey, A;Sohal, RS
认知和运动功能与年龄相关的损伤是由于氧化分子损伤的假设在小鼠中进行了测试。在一项盲法研究中，衰老小鼠（22个月大）接受了一系列运动和认知功能的行为测试，随后通过大脑不同区域的蛋白质羰基浓度评估氧化分子损伤。每只小鼠的年龄相关损伤程度是通过与参照组（4个月大）同时进行行为电池测试来确定的。研究发现，与年龄相关的空间游泳迷宫任务能力丧失与大脑皮层氧化分子损伤呈正相关，而与年龄相关的运动协调能力丧失与小脑氧化分子损伤相关。这些结果支持氧化应激是脑衰老的一个原因的观点。此外，研究结果表明，与年龄相关的认知和运动能力下降是独立进行的，并涉及大脑不同区域的氧化分子损伤。
The hypothesis that age-associated impairment of cognitive and motor functions is due to oxidative molecular damage was tested in the mouse. In a blind study, senescent mice (aged 22 months) were subjected to a battery of behavioral tests for motor and cognitive functions and subsequently assayed for oxidative molecular damage as assessed by protein carbonyl concentration in different regions of the brain. The degree of age-related impairment in each mouse was determined by comparison to a reference group of young mice (aged 4 months) tested concurrently on the behavioral battery. The age-related loss of ability to perform a spatial swim maze task was found to be positively correlated with oxidative molecular damage in the cerebral cortex, whereas age-related loss of motor coordination was correlated with oxidative molecular damage within the cerebellum. These results support the view that oxidative stress is a causal factor in brain senescence. Furthermore, the findings suggest that age-related declines of cognitive and motor performance progress independently, and involve oxidative molecular damage within different regions of the brain.