Heterochromatic differentiation in barley chromosomes revealed by C- and N-banding techniques
Heterochromatic differentiation in barley chromosomes revealed by C- and N-banding techniques
复制标题
C 和 N 带技术揭示大麦染色体的异染色质分化
DOI：
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发表时间：
1991
影响因子：
5.4
通讯作者：
H. Yamagata
中科院分区：
文献类型：
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作者：
K. Kakeda;K. Fukui;H. Yamagata
摘要通过分析4个品种的C带和n带，研究了大麦染色体中异染色质的分布。采用酶浸法和风干法制备染色体玻片。虽然这两种带型大体相似，但在所有染色体的着丝点上观察到它们之间的明显差异。每个品种的着丝点均由N带阳性和C带阴性（N+ C−）异染色质组成。在不同的显带技术中证实了异染色质分布的品种间多态性。部分条带的出现频率在两种条带技术之间和品种之间存在差异。讨论了观察到的异色分化的原因。
SummaryHeterochromatin distribution in barley chromosomes was investigated by analyzing the C- and N-banding patterns of four cultivars. Enzymatic maceration and air drying were employed for the preparation of the chromosome slides. Although the two banding patterns were generally similar to each other, a clear difference was observed between them at the centromeric sites on all chromosomes. Every centromeric site consisted of N-banding positive and C-banding negative (N+ C−) heterochromatin in every cultivar examined. An intervarietal polymorphism of heterochromatin distribution was confirmed in each of the banding techniques. The appearance frequencies of some bands were different between the two banding techniques and among the cultivars. The heterochromatic differentiation observed is discussed with respect to cause.