Effects of two different probiotics on microflora, morphology, and morphometry of gut in organic laying hens
Effects of two different probiotics on microflora, morphology, and morphometry of gut in organic laying hens
复制标题
DOI：
10.3382/ps/pew164
复制
发表时间：
2016-11-01
期刊：
影响因子：
4.4
通讯作者：
Franciosini, M. P.
中科院分区：
文献类型：
--
作者：
Forte, C.;Acuti, G.;Franciosini, M. P.
本研究探讨了嗜酸乳杆菌和枯草芽孢杆菌作为益生菌对有机蛋鸡肠道菌群、形态学和形态计量学的影响。将180只海兰蛋鸡分为3个同质组，从16至20周龄接受预沉积饮食，并在剩余的7个月的实验中接受沉积饮食。对照组（CTR）饲喂玉米-豆粕型日粮，第二组（L）在相同的日粮中添加0.1%的L。第三组（B）在基础日粮中添加0.05%的B。枯草芽孢杆菌在18周龄（T1）和沉积开始后5个月（T2）和7个月（T3），每组9名受试者被人道处死，用于肠道的微生物、形态学和形态测定分析。2种益生菌补充的饮食增加了乳酸杆菌属。和双歧杆菌属与CTR饮食相比。E. L组大肠杆菌、大肠菌群、葡萄球菌检出率均高于对照组（P < 0.001）。梭菌属L组和B组均降低（P < 0.001）。益生菌补充剂似乎会影响肠道微生物种群，促进有益细菌如乳酸杆菌的存在。和双歧杆菌属并减少潜在的有害细菌如E.大肠杆菌、梭菌和葡萄球菌。形态学和形态测量学分析未发现组间存在实质性差异。在T3，CTR母鸡绒毛中的浆细胞浸润比L和B组中观察到的更严重（P = 0.009）。
The current study investigated the effects of Lactobacillus acidophilus and Bacillus subtilis, used as probiotics, on the microflora, morphology, and morphometry of the gut in organic laying hens. The birds (180 Hy-Line laying hens) were divided into 3 homogenous groups and received a pre-deposition diet from 16 to 20 wk of age and a deposition diet for the remaining 7 months of the experiment. The control group (CTR) was fed a corn-soybean cake-based diet, the second group (L) received the same diet supplemented with 0.1% of L. acidophilus while in the third group (B) the basal diet was supplemented with 0.05% of B. subtilis. At 18 wk of age (T1) and at 5 (T2) and 7 months (T3) from the beginning of deposition, 9 subjects per group were humanely killed for microbiological, morphological and morphometric analyses of the intestinal tract. The 2 probiotic-supplemented diets increased Lactobacillus spp. and Bifidobacterium spp. counts compared with the CTR diet. The lowest viable counts of E. coli, coliforms and staphylococci were observed in the L group (P < 0.001). Clostridium spp. decreased (P < 0.001) in both L and B subjects. The probiotic supplementation appeared to affect the intestinal microbial population, promoting the presence of beneficial bacteria such as Lactobacillus spp. and Bifidobacterium spp. and reducing potential harmful bacteria such as E. coli, clostridia and staphylococci. Morphological and morphometric analyses did not reveal substantial differences among groups. At T3, the plasma cell infiltrate in the villi of the CTR hens was more severe than that observed in the L and B groups (P = 0.009).