Effect of hangekobokuto for amelioration of aggressiveness and social behavior in socially isolated mice.
Effect of hangekobokuto for amelioration of aggressiveness and social behavior in socially isolated mice.
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DOI：
10.1002/tkm2.1095
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Hu, A. L.
中科院分区：
文献类型：
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作者：
Harada, Yoshinao;Yamaguchi, Takuji;Hu, A. L.
目的：汉方药Hangekobokuto被认为对焦虑症、健忘症、失眠和神经性胃炎有效。然而，其作用机制和效果验证仍有待确认。因此，本研究的目的是在小鼠应激模型中确认hangekobukuto对改善攻击性和社会行为的效果。方法：4周龄雄性ddY小鼠通过隔离饲养4周的社会应激。对照组是在社会群体中长大的。然后，以0%（纯净水）、0.25%和0.5%的剂量在饮用水中向隔离饲养的小鼠经口给予Hangekobokuto，持续2周。社会抚养的对照组没有接受任何治疗。攻击性和社会行为进行了评估。试验结束后取血，测定血清皮质酮和白细胞介素（IL）-6。摘除大脑的前额区和脑干区，测定血清素、多巴胺和IL-6。结果如下：Hangekobokuto剂量依赖性地抑制隔离应激诱导的攻击行为，5 HT 1A受体拮抗剂处理后该作用消失。血皮质酮，隔离应激增加，降低hangekobukuto治疗。前额叶区的5-羟色胺和多巴胺，减少了隔离压力，增加了hangekobukuto治疗。脑干中的IL-6，隔离应激增加，hangekobokuto治疗剂量依赖性降低。结论：半言术能改善由应激引起的焦虑引起的攻击性。此外，作用机制涉及血清素系统。
Aim: Hangekobokuto, a kampo medicine, is considered effective against anxiety neurosis, amnesia, insomnia, and neurotic gastritis. The mechanism of action and verification of effect, however, remain to be confirmed. The aim of this study was therefore to confirm the effect of hangekobokuto for amelioration of aggressiveness and social behavior in a mouse stress model. Methods: Four-week-old male ddY mice were socially stressed by isolated rearing for 4 weeks. The control group was reared in a social group. Hangekobokuto was then given orally in drinking water to the isolation-reared mice at a dose of 0% (purified water), 0.25% and 0.5% for 2 weeks. The socially reared control group was not given any treatment. Aggressiveness and social behavior were evaluated. Blood samples were obtained after the test, and serum corticosterone and interleukin (IL)-6 were measured. The prefrontal region of the brain and the brainstem region were enucleated, and serotonin, dopamine and IL-6 were measured. Results: Hangekobokuto dose-dependently controlled the aggressive behavior induced by isolation stress, and the effect disappeared on 5HT1A receptor antagonist treatment. Blood corticosterone, increased by isolation stress, decreased with hangekobokuto treatment. Serotonin and dopamine in the prefrontal region, decreased by isolation stress, increased with hangekobokuto treatment. IL-6 in the brainstem, increased by isolation stress, decreased dose dependently on hangekobokuto treatment. Conclusion: Hangekobokuto ameliorates aggressiveness induced by anxiety caused by stress. Furthermore, the mechanism of effect involves the serotonin system.