Metabolic profiling of rodent biological fluids via 1H NMR spectroscopy using a 1 mm microlitre probe
Metabolic profiling of rodent biological fluids via 1H NMR spectroscopy using a 1 mm microlitre probe
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作者：
Griffin, JL;Nicholls, AW;Kuehn, T
本文介绍了一种1 mm TXI（H-1/C-13/N-15）微升核磁共振探针在生物流体代谢谱分析中的应用。该探针用于提供大鼠血浆的光谱分布，仅使用类似于2穆尔的液体，并采用一系列溶剂抑制技术。使用类似量的流体，从大鼠和小鼠脑脊液中获得光谱，表明该探针可用于通过以前无法进行NMR分析的生物流体来分析啮齿动物的代谢，而无需终止。
The application of a 1 mm TXI (H-1/C-13/N-15) microlitre NMR probe with z-gradient for metabolic profiling of biofluids is described. The probe was used to provide spectral profiles for rat blood plasma using only similar to2 mul of fluid with a range of solvent suppression techniques. Using a similar amount of fluid, spectra were obtained from rat and mouse cerebrospinal fluid, demonstrating that the probe could be used to profile rodents metabolically via biofluids previously inaccessible to NMR analysis without the need for termination.