The barley genome sequence assembly reveals three additional members of the CslF (1,3;1,4)-β-glucan synthase gene family.
The barley genome sequence assembly reveals three additional members of the CslF (1,3;1,4)-β-glucan synthase gene family.
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DOI：
10.1371/journal.pone.0090888
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Halpin C
中科院分区：
文献类型：
--
作者：
Schreiber M;Wright F;MacKenzie K;Hedley PE;Schwerdt JG;Little A;Burton RA;Fincher GB;Marshall D;Waugh R;Halpin C
大麦细胞壁的一个重要成分，特别是胚乳中的一个重要成分，是（1，3; 1，4）-β-葡聚糖，这是一种聚合物，已被证明对人类健康有益，并影响酿造工业的加工性能。纤维素酶样（Csl）F基因家族的基因已被证明参与（1，3; 1，4）-β-葡聚糖的合成，但生物合成的许多方面仍不清楚。大麦基因组序列组装的检查揭示了另外三个HvCslF基因（HvCslF 11、HvCslF 12和HvCslF 1, 3）的存在，其可能参与（1，3; 1，4）-β-葡聚糖合成。HvCslF 11和HvCslF 12 mRNA的转录本分别在根和幼叶中发现。这些基因在本氏烟草（Nicotiana benthamiana）中的瞬时表达导致浸润叶的表型变化，尽管没有检测到真正的（1，3; 1，4）-β-葡聚糖。谷物中CslF基因家族的比较揭示了基因间重组、基因复制和易位事件的证据。该基因家族内的这种显著差异可能与禾本科植物中（1，3; 1，4）-β-葡聚糖的多种功能有关。大麦和其他谷物的基因组和全球表达数据是表征完整基因家族进化和动态的强大资源。在CslF基因家族的情况下，结果将有助于更透彻地了解草细胞壁中的碳水化合物代谢。
An important component of barley cell walls, particularly in the endosperm, is (1,3;1,4)-β- glucan, a polymer that has proven health benefits in humans and that influences processability in the brewing industry. Genes of the cellulose synthase-like (Csl) F gene family have been shown to be involved in (1,3;1,4)-β-glucan synthesis but many aspects of the biosynthesis are still unclear. Examination of the sequence assembly of the barley genome has revealed the presence of an additional three HvCslF genes (HvCslF11, HvCslF12 and HvCslF13) which may be involved in (1,3;1,4)-β-glucan synthesis. Transcripts of HvCslF11 and HvCslF12 mRNA were found in roots and young leaves, respectively. Transient expression of these genes in Nicotiana benthamiana resulted in phenotypic changes in the infiltrated leaves, although no authentic (1,3;1,4)-β-glucan was detected. Comparisons of the CslF gene families in cereals revealed evidence of intergenic recombination, gene duplications and translocation events. This significant divergence within the gene family might be related to multiple functions of (1,3;1,4)-β-glucans in the Poaceae. Emerging genomic and global expression data for barley and other cereals is a powerful resource for characterising the evolution and dynamics of complete gene families. In the case of the CslF gene family, the results will contribute to a more thorough understanding of carbohydrate metabolism in grass cell walls.