Genome-wide linkage analysis of lipids in nondiabetic chinese and Japanese from the SAPPHIRe family study
Genome-wide linkage analysis of lipids in nondiabetic chinese and Japanese from the SAPPHIRe family study
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DOI：
10.1016/j.amjhyper.2006.05.004
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发表时间：
2006-12-01
影响因子：
3.2
通讯作者：
Hsiung, Chao A.
中科院分区：
文献类型：
--
作者：
Hsiao, Chin-Fu;Chiu, Yen-Feng;Hsiung, Chao A.
背景：血脂水平被认为是冠心病的主要危险因素。方法：我们对1538名中国受试者(509个家系)和625名日本受试者(204个家系)进行了与血脂表型相关的数量性状基因座全基因组研究。结果：中国受试者12号染色体113 cM处的对数转化的高密度脂蛋白-胆固醇的最高对数值(LOD)得分为3.22。这一分数与墨西哥裔美国人的高密度脂蛋白阳性结果(第12号染色体长96厘米)重叠。虽然在日本受试者中没有发现明显的关联证据，但在其他已发表的基因组扫描中已报道的几个区域中发现了一些适度的峰值(LOD分数=1.5)。例如，日本人的高密度脂蛋白的蓝宝石峰(染色体I位于167cM；LOD=1.54)与美国白种人HyperGEN(1号染色体位于159.9 cM)扫描中报告的高密度脂蛋白的数量性状座位非常接近。结论：在全基因组扫描中，我们发现在中国受试者中，高密度脂蛋白与12号染色体上的一个座位存在显著连锁，日本受试者中未发现超过1.68的连锁信号。
Background: Lipid levels are recognized as major risk factors for coronary heart disease (CHD). Discovery of major loci underlying quantitative lipid traits could help to elucidate the genetics of CHD.Methods: We performed a genome-wide search for quantitative trait loci linked to lipid phenotypes in 1538 Chinese subjects (509 families) and 625 Japanese subjects (204 families) not taking lipid-lowering medications from the Stanford-Asian Pacific Program in Hypertension and Insulin Resistance (SAPPHIRe) study. The multipoint variance-components method was used to test for linkage between marker loci and each trait by maximum likelihood methods adjusted for effects of age, age, gender, body mass index (BMI), smoking, alcohol drinking, physical activity, and field center.Results: The highest logarithm of odds (LOD) score detected was 3.22 for logarithmically transformed HDL-cholesterol on chromosome 12 at 113 cM in Chinese subjects. This score overlaps the positive findings for HDL reported in Mexican Americans (chromosome 12 at 96 cM). Although no strong evidence for linkage was found in Japanese subjects, some modest peaks (LOD score >= 1.5) were found in several regions that have been reported in other published genome scans. For example, the Japanese SAPPHIRe peak for HDL (chromosome I at 167 cM; LOD = 1.54) was very close to the quantitative trait loci (QTL) for HDL reported in the scan of white American HyperGEN (chromosome 1 at 159.9 cM).Conclusions: Genome-wide scan for genes influencing lipid phenotypes was conducted and we found significant linkage of HDL to a locus on chromosome 12 in Chinese subjects and no linkage signal exceeding 1.68 was found in the Japanese subjects.