A poplar R2R3-MYB transcription factor, PtrMYB152, is involved in regulation of lignin biosynthesis during secondary cell wall formation
A poplar R2R3-MYB transcription factor, PtrMYB152, is involved in regulation of lignin biosynthesis during secondary cell wall formation
复制标题
杨树 R2R3-MYB 转录因子 PtrMYB152 参与次生细胞壁形成过程中木质素生物合成的调节
DOI：
10.1007/s11240-014-0555-8
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发表时间：
2014-12-01
影响因子：
3
通讯作者：
Luo, Keming
中科院分区：
文献类型：
--
作者：
Li, Chaofeng;Wang, Xianqiang;Luo, Keming
木材是多年生木本植物中最丰富的生物量。大量研究表明，R2 R3-MYB转录因子参与了拟南芥、水稻、玉米和白杨等植物次生细胞壁形成过程中木质素生物合成的调控。本研究从大果杨中分离到一个MYB转录因子PtrMYB 152，它编码一个321个氨基酸的蛋白质，含有一个保守的R2 R3-MYB结构域。系统发育分析表明，PtrMYB 152与已知的与次生壁形成相关的MYB基因具有高度的同源性，PtrMYB 152在白杨次生壁形成细胞中特异表达。GUS组织化学定位结果表明，PtrMYB 152主要在维管组织中表达。PtrMYB 152的过量表达导致木质素合成基因的特异性激活，并导致木质素在转基因植株的茎和叶柄中异位沉积。说明PtrMYB 152是白杨木材形成过程中木质素合成的特异性转录激活因子。
Wood is the most abundant biomass in perennial woody plants. Extensive studies have shown that R2R3-MYB transcription factors are involved in regulation of lignin biosynthesis during secondary cell wall formation in many plant species, such asArabidopsis, rice, maize and poplar. In this study, a MYB transcription factor, namedPtrMYB152, was isolated fromPopulus trichocarpaand encoded a protein of 321 amino acids that contained a conserved R2R3-MYB domain. Phylogenetic analysis revealed that PtrMYB152 shares high sequence homology with other known plant MYBs associated with secondary wall formation.PtrMYB152is specifically expressed in secondary wall-forming cells in poplar. Histochemical localizations of GUS expression in the transgenicArabidopsisplants showed thatPtrMYB152is predominantly expressed in vascular tissues. Overexpression ofPtrMYB152resulted in specific activation of lignin biosynthetic genes, and caused ectopic deposition of lignin in stem and petiole of transgenic plants. These data indicated that PtrMYB152 is a specific transcriptional activator of lignin biosynthesis during wood formation of poplar.