Multi-site Analysis Reveals Widespread Antibiotic Resistance in the Marine Pathogen Vibrio vulnificus
Multi-site Analysis Reveals Widespread Antibiotic Resistance in the Marine Pathogen Vibrio vulnificus
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DOI：
10.1007/s00248-008-9413-8
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发表时间：
2009-01-01
影响因子：
3.6
通讯作者：
Stepanauskas, Ramunas
中科院分区：
文献类型：
--
作者：
Baker-Austin, Craig;McArthur, J. V.;Stepanauskas, Ramunas
创伤弧菌是一种严重的机会性人类病原体，常见于亚热带沿海水域，是美国海鲜传播死亡的主要原因。该分类单元不能在临床或农业环境中长期存在，因为它会经历人类诱导的抗生素抗性选择。因此，很少有研究验证常用抗生素治疗创伤弧菌的有效性。在这里，我们针对代表不同作用模式的 26 种抗菌药物筛选了 151 种沿海分离株和 10 种原发性败血症分离株。对各种来源的抗生素产生多重耐药性的频率出乎意料地高，特别是在夏季，并且相当大比例的分离株（17.3%）对八种或更多抗菌药物具有耐药性。许多分离株表现出对治疗创伤弧菌感染的常规抗生素（如多西环素、四环素、氨基糖苷类和头孢菌素）的耐药性。这些耐药性在强毒株和非强毒株（基于 PCR 的毒力分型）中检测到的频率相似，并且存在于败血症分离株中，这是我们的研究结果对公共卫生的影响。在环境分离株中，原始河口和受人为影响的河口之间的抗性频率没有一致的差异，这表明抗性特征的来源是自然的，而不是人为的。该报告首次证明了没有临床储存库的人类病原体中普遍存在抗生素耐药性，这意味着环境研究在了解抗生素耐药性因素的传播、进化和公共卫生相关性方面的重要性。
Vibrio vulnificus is a serious opportunistic human pathogen commonly found in subtropical coastal waters, and is the leading cause of seafood-borne mortality in the USA. This taxon does not sustain prolonged presence in clinical or agricultural settings, where it would undergo human-induced selection for antibiotic resistance. Therefore, few studies have verified the effectiveness of commonly prescribed antibiotics in V. vulnificus treatment. Here we screened 151 coastal isolates and 10 primary septicaemia isolates against 26 antimicrobial agents representing diverse modes of action. The frequency of multiple resistances to antibiotics from all sources was unexpectedly high, particularly during summer months, and a substantial proportion of isolates (17.3%) were resistant to eight or more antimicrobial agents. Numerous isolates demonstrated resistance to antibiotics routinely prescribed for V. vulnificus infections, such as doxycycline, tetracycline, aminoglycosides and cephalosporins. These resistances were detected at similar frequencies in virulent and non-virulent strains (PCR-based virulence typing) and were present in septicaemia isolates, underlying the public health implications of our findings. Among environmental isolates, there were no consistent differences in the frequency of resistance between pristine and anthropogenically impacted estuaries, suggesting natural rather than human-derived sources of resistance traits. This report is the first to demonstrate prevalent antibiotic resistance in a human pathogen with no clinical reservoirs, implying the importance of environmental studies in understanding the spread, evolution and public health relevance of antibiotic resistance factors.