DIFFERENTIAL BINDING-CAPACITY AND INTERNALIZATION OF BACTERIAL SUBSTRATES AS FACTORS IN GROWTH-RATE OF ACANTHAMOEBA SPP
DIFFERENTIAL BINDING-CAPACITY AND INTERNALIZATION OF BACTERIAL SUBSTRATES AS FACTORS IN GROWTH-RATE OF ACANTHAMOEBA SPP
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DOI：
10.1099/00222615-40-2-148
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发表时间：
1994-02-01
影响因子：
3
通讯作者：
QURESHI, MN
中科院分区：
文献类型：
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作者：
BOTTONE, EJ;PEREZ, AA;QURESHI, MN
棘阿米巴是一种自由生活的捕食者，有选择地以细菌为食。细菌食物源粘附到滋养体膜上，随后是内化和消化。通过与嗜麦芽黄单胞菌、大肠杆菌、表皮葡萄球菌和铜绿假单胞菌（尽管后者具有杀阿米巴的特性）共同培养castellanii和A，来判断首选细菌底物的特异性。嗜麦芽假单胞菌和铜绿假单胞菌几乎立即粘附在一起，在这两种变形虫的滋养体周围形成一个多层的芽孢杆菌套。大肠杆菌粘附在滋养体上的数量较少，数量也较少。嗜麦芽葡萄球菌在共培养8小时后很容易内化，而铜绿假单胞菌、大肠杆菌和表皮葡萄球菌即使在共培养24小时后也没有内化。这些数据表明，细菌食物来源对棘阿米巴属的适宜性不仅与细菌与滋养体表面结合的倾向有关，而且与其内化的速度有关。
Acanthamoeba spp. are free-living predators that selectively feed on bacteria. Adherence of the bacterial food source to the trophozoite membrane is followed by internalisation and digestion. Through co-cultivation of A. castellanii and A, polyphaga, individually, with Xanthomonas maltophilia, Escherichia coli, Staphylococcuss epidermidis and Pseudomonas aeruginosa (despite the amoebicidal properties of the latter organism), specificity with regard to the preferred bacterial substrate was judged. X. maltophilia and P. aeruginosa adhered almost immediately forming a multilayered mantle of bacilli around trophozoites of both species of amoebae. E. coli adhered to fewer trophozoites and in smaller numbers. X. maltophilia was readily internalised after co-cultivation for 8 h, whereas P. aeruginosa, E. coli and S. epidermidis were not internalised even after co-cultivation for 24 h. These data suggest that the suitability of a bacterial food source for the Acanthamoeba spp. studied is associated not only with the proclivity with which the bacterial species binds to the trophozoite surface, but also with the rate of its internalisation.