Slantingly cross loading sample system enables simultaneous performance of separation and mixture to detect molecular interactions on thin-layer chromatography
Slantingly cross loading sample system enables simultaneous performance of separation and mixture to detect molecular interactions on thin-layer chromatography
复制标题
DOI：
10.1016/j.chroma.2012.05.011
复制
发表时间：
2012-07-06
影响因子：
4.1
通讯作者：
Nakayama, Masayoshi
中科院分区：
文献类型：
--
作者：
Shimizu-Yumoto, Hiroko;Hayashi, Nobuyuki;Nakayama, Masayoshi
花色素苷是主要的花色素，可以受到辅色素的影响，辅色素是无色的化合物，可以将花色素苷的颜色修改为更强烈和更蓝。薄层色谱法是分离和分析花色苷和辅色素的有效方法。为了方便、全面地检测混合色素，我们在TLC中增加了混合功能，通过倾斜交叉上样，使混合色素在从上原点到各Rf值的不同角度线上对称展开，并有序交叉，混合与分离同时进行。在花色素苷的显色线上，可以检测到辅色素的发生。通过交叉薄层色谱法分析粉红色香豌豆（LathyrusodoratusL）花瓣，并且在花青苷线上检测到更强烈的斑点和更浅的斑点。由于每个斑点与紫外吸收线重叠，这些紫外吸收化合物中的每一种被纯化并分别鉴定为山奈酚3-鼠李糖苷和2-氰乙基-异恶唑啉-5-酮。山奈酚3-鼠李糖苷是一种黄酮类化合物，具有更强烈和更蓝的着色变化的一般复制色素效应，而2-氰乙基-异恶唑啉-5-酮是一种结构超出常规复制色素类别的化合物，具有将花色苷着色变得更浅的新效果，同时保持色调。我们确定，应在没有预先存在的结构和效应预测的情况下进行对复制色素的搜索。我们已经证明，在板式色谱上的倾斜交叉加载样品系统是一种有效的技术，用于这种全面的分析分子相互作用。皇冠版权所有（c）2012由爱思唯尔B. V.出版保留所有权利。
Anthocyanins are major flower pigments that can be affected by copigments, colorless compounds that can modify anthocyanin coloration to more intense and bluer. Thin-layer chromatography (TLC) is an available technique to separate and analyze anthocyanins and copigments. To easily and comprehensively detect copigments, we added function of mixture of compounds to TLC; by slantingly cross loading samples on TLC, compounds are symmetrically developed at various angle lines from the upper origin to individual R-f values and cross each other in an orderly fashion, where mixture is simultaneously performed with separation. Occurrence of copigments can be detected as a coloration change on the developed line of anthocyanin. Pink sweet pea (Lathyrus odoratus L) petals were analyzed by the cross-TLC and a more intense spot and a paler spot on the anthocyanin line were detected. As each spot overlapped with an ultraviolet absorbance line, each of these ultraviolet absorption compounds was purified and identified as kaempferol 3-rhamnoside and 2-cyanoethyl-isoxazolin-5-one, respectively. Whereas kaempferol 3-rhamnoside is a flavonoid and had a general copigment effect of more intense and bluer coloration change, 2-cyanoethyl-isoxazolin-5-one is a compound whose structure is outside of conventional categories of copigments and had a novel effect to change anthocyanin coloration paler while maintaining color tone. We determined that the search for copigments should be carried out without pre-existing prediction of structures and effects. We have shown that slantingly cross loading samples system on plate-type chromatography is an effective technique for such comprehensive analysis of molecular interaction. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.