Conventional and molecular determination of drug resistance in Mycobacterium tuberculosis and Mycobacterium bovis isolates in cattle
Conventional and molecular determination of drug resistance in Mycobacterium tuberculosis and Mycobacterium bovis isolates in cattle
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DOI：
10.1016/j.tube.2018.12.005
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发表时间：
2019-01-01
期刊：
影响因子：
3.2
通讯作者：
Thangavelu, A.
中科院分区：
文献类型：
--
作者：
Anne, N. Sweetline;Ronald, B. S. M.;Thangavelu, A.
本研究采用常规微量肉汤稀释法和分子方法，对51株牛源结核分枝杆菌和牛分枝杆菌的耐药情况进行了研究。用微量肉汤稀释法检测，异烟肼耐药16株，吡嗪酰胺耐药08株，利福平耐药09株，乙胺丁醇耐药07株。2株对利福平和吡嗪酰胺耐药，1株对吡嗪酰胺和乙胺丁醇耐药，03株对异烟肼和乙胺丁醇耐药。所有分离株均未出现多药耐药（MDR）。除牛支原体菌株外，其他结核分枝杆菌分离株均未表现出对吡嗪酰胺的耐药性。分子方法采用多重PCR靶向katG、pncA、rpoB基因，多重等位基因特异性PCR检测embB密码子306突变，测序结果显示katG和rpoB基因点突变。多重等位基因特异性PCR未检测到embB基因突变。这些结果表明，由于牛中存在耐药结核分枝杆菌，这可能是由于结核病流行地区的人类传播而导致的，这使得结核病根除计划更具挑战性，因此需要进行进一步的详细研究。
This study was aimed out to explore the presence of drug resistance among M. tuberculosis and M. bovis isolates (n = 51) of bovine origin by conventional broth microdilution method and molecular methods. By broth microdilution method, 16 isolates were found to be resistant to isoniazid, 08 isolates were resistant to pyrazinamide, 09 isolates were resistant to rifampicin and 07 isolates were found to be resistant for ethambutol. Two isolates showed resistance to rifampicin and pyrazinamide, one isolate showed resistance to pyrazinamide and ethambutol and 03 isolates showed resistance to isoniazid and ethambutol. None of isolates showed multi drug resistance (MDR). Other than the M. bovis strains, none of the other M. tuberculosis isolates showed any resistance to pyrazinamide. Molecular methods by multiplex PCR targeting katG, pncA, rpoB genes, multiplex allele specific PCR to detect mutation in embB codon 306 and sequencing showed point mutation in katG and rpoB gene. No mutation could be detected in the embB gene by multiplex allele specific PCR. The results indicates that further elaborate studies need to be carried out due to the presence of drug resistant M. tuberculosis in bovines which could be due to spill over from human in tuberculosis endemic areas making TB eradication programme more challenging.