Diversity of Antibody Responses to Borrelia burgdorferi in Experimentally Infected Beagle Dogs
Diversity of Antibody Responses to Borrelia burgdorferi in Experimentally Infected Beagle Dogs
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DOI：
10.1128/cvi.00018-14
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发表时间：
2014-06-01
影响因子：
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通讯作者：
Barbour, Alan G.
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文献类型：
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作者：
Baum, Elisabeth;Grosenbaugh, Deborah A.;Barbour, Alan G.
莱姆病（LB）是一种常见的感染，家犬在地方性传播的代理伯氏疏螺旋体。虽然基于单个亚单位的免疫测定法已在很大程度上取代了犬血清学全细胞制剂的使用，但仅确定了有限数量的信息性抗原。为了更广泛地表征对B的抗体应答。为了评估个体狗中这些反应的多样性，我们检测了32只成年群体繁殖的比格狗的血清，这些狗已经实验性地感染了B。并将这些血清在蛋白质微阵列中与来自未感染狗的血清在它们对各种重组染色体和质粒编码的B的抗体反应性方面进行比较。burgdorferi蛋白，包括24种北美的定义型的OspC蛋白。犬的免疫原性蛋白质谱与人类和天然储库啮齿动物的免疫原性蛋白质谱基本相似;这些蛋白质包括核心蛋白聚糖结合蛋白DbpB、BBA 36、BBA 57、BBA 64、纤连蛋白结合蛋白BBK32、VlsE、FlaB和其他鞭毛结构蛋白、Erp蛋白、Bdr蛋白和所有OspC蛋白。此外，犬血清与假定的脂蛋白BBB 14和BB 0844结合，这在人类或啮齿动物中很少引起抗体。虽然比格犬，像大多数其他家犬品种，具有小的有效人口规模和功能广泛的连锁不平衡，在这里研究的动物组表现出多样性的抗体反应的措施，抗体水平和特异性保守的蛋白质，如DbpB，和多态性蛋白质，如OspC。
Lyme borreliosis (LB) is a common infection of domestic dogs in areas where there is enzootic transmission of the agent Borrelia burgdorferi. While immunoassays based on individual subunits have mostly supplanted the use of whole-cell preparations for canine serology, only a limited number of informative antigens have been identified. To more broadly characterize the antibody responses to B. burgdorferi infection and to assess the diversity of those responses in individual dogs, we examined sera from 32 adult colony-bred beagle dogs that had been experimentally infected with B. burgdorferi through tick bites and compared those sera in a protein microarray with sera from uninfected dogs in their antibody reactivities to various recombinant chromosome and plasmid-encoded B. burgdorferi proteins, including 24 serotype-defining OspC proteins of North America. The profiles of immunogenic proteins for the dogs were largely similar to those for humans and natural-reservoir rodents; these proteins included the decorin-binding protein DbpB, BBA36, BBA57, BBA64, the fibronectin-binding protein BBK32, VlsE, FlaB and other flagellar structural proteins, Erp proteins, Bdr proteins, and all of the OspC proteins. In addition, the canine sera bound to the presumptive lipoproteins BBB14 and BB0844, which infrequently elicited antibodies in humans or rodents. Although the beagle, like most other domestic dog breeds, has a small effective population size and features extensive linkage disequilibrium, the group of animals studied here demonstrated diversity in antibody responses in measures of antibody levels and specificities for conserved proteins, such as DbpB, and polymorphic proteins, such as OspC.