Expression of a member of tumor necrosis factor receptor superfamily, TROY, in the developing olfactory system
Expression of a member of tumor necrosis factor receptor superfamily, TROY, in the developing olfactory system
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作者：
Hisaoka, T;Morikawa, Y;Senba, E
TROY是最近发现的肿瘤坏死因子（TNF）受体超家族成员。我们以前曾报道过，TROY通过TNF受体相关因子2、5和6诱导核因子κ B的活化，并在发育中的中枢神经系统（包括嗅球）中强烈表达。在这项研究中，我们研究了详细的细胞特性的TROY表达细胞在发展中的嗅觉系统的小鼠使用原位杂交和免疫组织化学。TROY的mRNA和蛋白在胚胎第13.5天的嗅球嗅神经层（ONL）中首次被检测到。在胚胎发育后期，TROY的表达是最强烈的内部ONL（ONL-i）。在出生后的嗅球中，除了ONL-i之外，还在肾小球层（GL）中检测到TROY表达细胞。免疫荧光双标法显示，ONL-i嗅鞘细胞（OECs）表达TROY，神经肽Y（NPY）阳性，S-100和p75低亲和力神经生长因子受体均不表达。在ONL-i中观察到一些表达TROY的细胞具有星形胶质细胞样表型（GFAP（+）/NPY-）。此外，在GL的GFAP（+）胶质细胞中也检测到TROY。因此，TROY在嗅球中的一些特定的胶质细胞亚群中表达，包括嗅鞘细胞，表明TROY可能在发育和成年嗅觉系统中发挥一定的作用。(C)2003 Wiley-Liss，Inc.
TROY is a recently identified member of the tumor necrosis factor (TNF) receptor superfamily. We have previously reported that TROY induces the activation of nuclear factor kappaB via TNF receptor-associated factor 2, 5, and 6, and is strongly expressed in the developing central nervous system, including the olfactory bulb. In this study, we investigated the detailed cellular characterization of TROY-expressing cells in the developing olfactory system of mice using in situ hybridization and immunohistochemistry. Both mRNA and protein of TROY were first detected in the olfactory nerve layer (ONL) of the olfactory bulb at embryonic day 13.5. During late embryogenesis, TROY expression was most intense in the inner ONL (ONL-i). In the postnatal olfactory bulb, TROY-expressing cells were also detected in the glomerular layer (GL), in addition to the ONL-i. The double-immunofluorescence method demonstrated that TROY was expressed in olfactory ensheathing cells (OECs) of the ONL-i, which were positive for neuropeptide Y (NPY), but neither S-100 nor p75 low-affinity nerve growth factor receptor. Some TROY-expressing cells in the ONL-i were observed with the astrocyte-like phenotype (GFAP(+)/NPY-). In addition, TROY was also detected in GFAP(+) glial cells of the GL. Thus, TROY was expressed in some specific subsets of glial cells in the olfactory bulb, including OECs, suggesting that TROY may play some roles in the developing and adult olfactory system. (C) 2003 Wiley-Liss, Inc.