Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP.
Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP.
复制标题
单克隆抗体 NM11 可识别 p300 和 CBP 共享的 C 末端表位。
DOI：
10.1089/hyb.1997.16.273
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Moran,E
中科院分区：
文献类型：
--
作者：
Dallas,PB;Yaciuk,P;Moran,E
The epitope recognized by the monoclonal antibody NM11, previously shown to recognize both CBP and p300, has been mapped here to the C-terminal third of p300 and CBP by Western analysis of p300 and CBP prokaryotic fusion proteins. More precise epitope mapping, carried out by screening a plasmid expression library derived from small randomly generated CBP cDNA fragments localizes the NM11 epitope to a 21 amino acid stretch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and p300 differ by three noncontiguous residues within this 21 amino acid region, a difference that does not detectably affect the reactivity of NM11.
