Early evaluation of renal medulla injury by 1H nuclear magnetic resonance spectroscopy after preservation and transplantation
Early evaluation of renal medulla injury by 1H nuclear magnetic resonance spectroscopy after preservation and transplantation
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DOI：
10.1016/s0041-1345(00)00853-8
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发表时间：
2000-03-01
影响因子：
0.9
通讯作者：
Hauet, T
中科院分区：
文献类型：
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作者：
Baumert, H;Richer, JP;Hauet, T
材料和方法我们使用大型白色猪。研究了三个实验组。我们比较了两种不同的冷藏解决方案，欧洲柯林斯（EC）和威斯康星州（UW）的解决方案。肾脏保存组取肾脏，用4C保存液500 mL冲洗.将肾脏包裹在含有500 mL保存液的无菌塑料袋中，并在融冰上储存48小时。将实验组与对照组（单侧肾切除）进行比较。在手术前和移植后第1、3、5、7、11和14天（D1至D14）通过肌酐清除率测定肾功能。还收集血液和尿液进行质子核磁共振（NMR）光谱分析，直到动物死亡或处死。对于尿NMR光谱，计算三甲基胺-N-氧化物（TMAO）与肌酸酐的比率，并以mmol/mol肌酸酐表示。报告的所有值均为平均值SEM，并通过方差分析和Student-Neuman Keuls检验进行多重比较。
MATERIALS AND METHODSWe used large white pigs. Three experimental groups were studied. We compared two different cold storage solutions, Euro–Collins (EC) and University of Wisconsin (UW) solutions. In the kidney preserved groups, kidneys were removed and ffushed with 500 mL of 4 C preservation solution. The kidney was wrapped in a sterile plastic bag containing 500 mL of preservation solution and stored for 48 hours on melting ice. Experimental groups were compared to a control group (uninephrectomized). Renal function was determined by creatinine clearance before surgery and after transplantation on days 1, 3, 5, 7, 11, and 14 (D1 to D14). Blood and urine were also collected for proton nuclear magnetic resonance (NMR) spectroscopy analysis until the deaths or sacrifice of the animals. For urine NMR spectra, the ratio of trimethylamine-N-oxide (TMAO) to creatinine was calculated and expressed in mmol/mol of creatinine. All values reported are means SEM and were compared by using variance analysis and a Student–Neuman Keuls test for multiple comparison.