Cloning and characterization of 60S ribosomal protein L22 (RPL22) from Culex pipiens pallens
Cloning and characterization of 60S ribosomal protein L22 (RPL22) from Culex pipiens pallens
复制标题
淡色库蚊 60S 核糖体蛋白 L22 (RPL22) 的克隆和表征。
DOI：
10.1016/j.cbpb.2009.03.003
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
--
作者：
He, Ji;Sun, Haibo;Zhu, Changliang
60S核糖体蛋白L22 (GenBank登录号：EF990190)克隆自淡色库蚊。在447 bps的开放阅读框（ORF）中编码了一个推测含有148个氨基酸的蛋白，该蛋白与埃及伊蚊和冈比亚按蚊的RPL22基因分别具有90%和80%的一致性。实时荧光定量PCR分析显示，抗性品系RPL22转录水平比敏感品系高2.57倍；pallens。RPL22在C6/36细胞中的过表达表明，C6/36-RPL22细胞对溴氰菊酯的抗性较对照降低。细胞色素P450 6A1 (CYP6A1， GenBank登录号：FJ423553)显示CYP6A1在RPL22转染的C6/36 （C6/36-RPL22）细胞中下调，表明CYP6A1受到RPL22的抑制。本研究首次证实RPL22基因可能参与淡色库蚊对溴氰菊酯的抗性调控。(C) 2009年Elsevier Inc.出版。
The 60S ribosomal protein L22 (GenBank accession no. EF990190) was cloned from Culex pipiens pallens. An open reading frame (ORF) of 447 bps was found to encode a putative 148 amino acids protein which shares 90% and 80% identity with RPL22 genes from Aedes aegypti and Anopheles gambiae respectively. Real-time quantitative PCR analysis demonstrated that the transcription level of RPL22 in deltamethrin-resistant strain was 2.57 folds higher than in deltamethrin-susceptible strain of Cx pipiens; pallens. Overexpression of RPL22 in C6/36 cells showed that the deltamethrin-resistance was decreased in C6/36-RPL22 cell compared to the control. The mRNA level of cytochrome P450 6A1 (CYP6A1, GenBank accession no. FJ423553) showed that CYP6A1 was down-regulated in the C6/36 transfected with RPL22 (C6/36-RPL22) cells, suggesting that CYP6A1 was repressed by RPL22. Our study provides the first evidence that RPL22 may play some role in the regulation of deltamethrin-resistance in Cx pipiens pallens. (C) 2009 Published by Elsevier Inc.