Titanium surface with nanospikes tunes macrophage polarization to produce inhibitory factors for osteoclastogenesis through nanotopographic cues
Titanium surface with nanospikes tunes macrophage polarization to produce inhibitory factors for osteoclastogenesis through nanotopographic cues
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DOI：
10.1016/j.actbio.2021.10.019
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发表时间：
2021-12-11
影响因子：
9.7
通讯作者：
Egusa, Hiroshi
中科院分区：
文献类型：
--
作者：
Kartikasari, Nadia;Yamada, Masahiro;Egusa, Hiroshi
确切预防种植体周围骨组织炎症性骨溶解仍未确定。M1巨噬细胞在宿主防御炎性骨溶解中发挥关键作用，其极化取决于细胞形状。巨噬细胞极化受环境刺激，特别是物理化学刺激的控制，因此纳米钛表面可能会调节巨噬细胞的极化和功能。本研究确定具有各向异性图案纳米尖的钛纳米表面是否调节巨噬细胞极化，从而抑制破骨细胞前体的破骨细胞分化。不同方案的碱蚀刻处理产生了两种类型的钛纳米表面，它们具有高或低分布密度的各向异性图案纳米尖，以及超亲水性和羟基的存在。在两种钛纳米表面上培养的J774A.1细胞（小鼠巨噬细胞样细胞系）表现出真正的循环形状和高表达的M1，但较少表达的M2，标记物，没有丧失活力。巨噬细胞在两种钛纳米表面上的m1样极化不依赖于蛋白质介导的配体刺激或钛表面的亲水性或化学状态。相比之下，其他光滑或微粗糙的钛表面很少或没有纳米尖刺，在任何培养条件下都不会激活巨噬细胞。两种钛纳米表面的巨噬细胞培养上清液即使与破骨细胞分化因子共孵育也能抑制RAW264.7细胞（小鼠破骨细胞前体细胞系）的破骨细胞分化。在纳米尖密度较大的钛纳米表面上培养的巨噬细胞对破骨细胞分化的抑制作用更强。这些结果表明，具有各向异性图案的纳米尖的钛纳米表面调节巨噬细胞极化，抑制破骨细胞前体的破骨细胞分化，其纳米形貌线索而不是其他物理化学性质。
Definitive prevention of inflammatory osteolysis around peri-implant bone tissue remains unestablished. M1 macrophages play a key role in the host defense against inflammatory osteolysis, and their polariza-tion depends on cell shape. Macrophage polarization is controlled by environmental stimuli, particularly physicochemical cues and hence titanium nanosurface might tune macrophage polarization and function. This study determined whether titanium nanosurfaces with anisotropically patterned nanospikes regu-lates macrophage polarization for inhibiting osteoclast differentiation of osteoclast precursors. Alkaline-etching treatment with different protocols created two types of titanium nanosurfaces that had anisotrop-ically patterned nanospikes with high or low distribution density, together with superhydrophilicity and the presence of hydroxyl groups. J774A.1 cells (mouse macrophage-like cell line), cultured on both ti-tanium nanosurfaces, exhibited truly circulated shapes and highly expressed M1, but less M2, mark-ers, without loss of viability. M1-like polarization of macrophages on both titanium nanosurfaces was independent of protein-mediated ligand stimulation or titanium surface hydrophilic or chemical sta-tus. In contrast, other smooth or micro-roughened titanium surfaces with little or no nanospikes did not activate macrophages under any culture conditions. Macrophage culture supernatants on both ti-tanium nanosurfaces inhibited osteoclast differentiation of RAW264.7 cells (mouse osteoclast precursor cell line), even when co-incubated with osteoclast differentiation factors. The inhibitory effects on osteo-clast differentiation tended to be higher in macrophages cultured on titanium nanosurfaces with denser nanospikes. These results showed that titanium nanosurfaces with anisotropically patterned nanospikes tune macrophage polarization for inhibiting osteoclast differentiation of osteoclast precursors, with nan-otopographic cues rather than other physicochemical properties.