Multiplexed miRNA fluorescence in situ hybridization for formalin-fixed paraffin-embedded tissues.
Multiplexed miRNA fluorescence in situ hybridization for formalin-fixed paraffin-embedded tissues.
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DOI：
10.1007/978-1-4939-1459-3_14
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发表时间：
2014
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影响因子：
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通讯作者：
Tuschl, Thomas
中科院分区：
文献类型：
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作者：
Renwick, Neil;Cekan, Pavol;Bognanni, Claudia;Tuschl, Thomas
多重miRNA荧光原位杂交（miRNA FISH）是一种先进的方法，用于可视化差异表达的miRNA，以及其他参考RNA，在存档组织。一些miRNA由于其丰度和细胞类型特异性而成为优秀的疾病生物标志物。然而，这些短RNA分子由于扩散损失、探针错杂交以及信号检测和信号放大问题而难以可视化。在这里，我们描述了一种可靠的和可调节的方法，用于在福尔马林固定的石蜡包埋（FFPE）组织切片中可视化和标准化miRNA信号。
Multiplexed miRNA fluorescence in situ hybridization (miRNA FISH) is an advanced method for visualizing differentially expressed miRNAs, together with other reference RNAs, in archival tissues. Some miRNAs are excellent disease biomarkers due to their abundance and cell-type specificity. However, these short RNA molecules are difficult to visualize due to loss by diffusion, probe mishybridization, and signal detection and signal amplification issues. Here, we describe a reliable and adjustable method for visualizing and normalizing miRNA signals in formalin-fixed paraffin-embedded (FFPE) tissue sections.