Hierarchy of Atg proteins in pre-autophagosomal structure organization
Hierarchy of Atg proteins in pre-autophagosomal structure organization
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DOI：
10.1111/j.1365-2443.2007.01050.x
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发表时间：
2007-02-01
期刊：
影响因子：
2.1
通讯作者：
Ohsumi, Yoshinori
中科院分区：
文献类型：
--
作者：
Suzuki, Kuninori;Kubota, Yuka;Ohsumi, Yoshinori
自噬是真核细胞中保守的大量降解过程，在细胞质物质和细胞器的周转中起作用。当真核细胞面临营养饥饿时，自噬体，一种双膜细胞器，由前自噬体结构（PAS）产生。在酿酒酵母中，16个ATG（autography-related）基因是自噬体形成所必需的。大多数Atg蛋白参与PAS，导致自噬体的产生。然而，考绩制度的组织机制仍有待阐明。在这里，我们进行了系统的和定量的分析，荧光显微镜开发的层次结构图参与PAS组织的Atg蛋白。该分析表明，Atg 17 p是PAS组织中最基本的蛋白质：当它特异性靶向质膜时，其他Atg蛋白被募集到该位置，这表明Atg 17 p作为支架蛋白将Atg蛋白组织到PAS。
Autophagy is a bulk degradation process that is conserved in eukaryotic cells and functions in the turnover of cytoplasmic materials and organelles. When eukaryotic cells face nutrient starvation, the autophagosome, a double-membraned organelle, is generated from the pre-autophagosomal structure (PAS). In the yeast Saccharomyces cerevisiae, 16 ATG (autography-related) genes are essential for autophagosome formation. Most of the Atg proteins are involved in the PAS, leading to autophagosome production. However, the mechanism of PAS organization remains to be elucidated. Here, we performed a systematic and quantitative analysis by fluorescence microscopy to develop a hierarchy map of Atg proteins involved in PAS organization. This analysis suggests that Atg17p is the most basic protein in PAS organization: when it is specifically targeted to the plasma membrane, other Atg proteins are recruited to that location, suggesting that Atg17p acts as a scaffold protein to organize Atg proteins to the PAS.