Local hemodynamics affect monocytic cell adhesion to a three-dimensional flow model coated with E-selectin
Local hemodynamics affect monocytic cell adhesion to a three-dimensional flow model coated with E-selectin
复制标题
DOI：
10.1016/s0021-9290(00)00139-1
复制
发表时间：
2001-01-01
影响因子：
2.4
通讯作者：
Alevriadou, BR
中科院分区：
文献类型：
--
作者：
Hinds, MT;Park, YJ;Alevriadou, BR
单核细胞对内皮的粘附取决于受体/配体的浓度、化学引诱剂的局部浓度、单核细胞向内皮表面的转运和血流动力学。单核细胞对三维灌注模型惰性表面的粘附被证明与壁剪切应力成反比，但也受到影响近壁细胞可用性的流动模式的影响。我们假设（a）在相同的流动条件下，与未涂覆的模型相比，模型表面上不溶的 E-选择素可能在较高的壁剪切应力下介导粘附相互作用，（b）脉动流可能会改变在稳定流动下获得的粘附分布。具有狭窄和突然扩张的轴对称流动模型产生了一系列壁剪切应力以及具有再循环和重新附着的分离流动。预激活的 U937 细胞在稳定 (Re = 100, 140) 或脉动 (Re-mean= 107) 流下通过模型进行灌注。速度场通过计算流体动力学进行表征，并通过惰性粒子跟踪进行验证。与相同流动条件下未包被的模型相比，表面 E-选择素在 Re = 100 和 140 时大大增加了所有区域的细胞粘附。在靠近壁的细胞丰富的区域（锥度和狭窄），当流量稳定时，对 E-选择素的粘附与局部壁剪切应力的倒数相关。 Pulsatile 现在将贴壁细胞更均匀地分布在整个涂层模型中。因此，表征血管壁上的局部血流动力学和生物活性对于白细胞粘附非常重要。 (C) 2000 Elsevier Science Ltd. 保留所有权利。
Monocyte adhesion to the endothelium depends on concentrations of receptors/ligands, local concentrations of chemoattractants, monocyte transport to the endothelial surface and hemodynamic forces. Monocyte adhesion to the inert surface of a three-dimensional perfusion model was shown to correlate inversely with wall shear stress, but was also affected by flow patterns which influenced the near-wall cell availability. We hypothesized that (a) under the same flow conditions, insolubilized E-selectin on the model's surface may mediate adhesive interactions at higher wall shear stresses, compared to an uncoated model, and (b) pulsatic flow may modify the adhesion profile obtained under steady flow. An axisymmetric flow model with a stenosis and a sudden expansion produced a range of wall shear stresses and a separated flow with recirculation and reattachment. Pre-activated U937 cells were perfused through the model under either steady (Re = 100, 140) or pulsatile(Re-mean= 107) flow. The velocity field was characterized through computational fluid dynamics and validated by inert particle tracking. Surface E-selectin greatly increased cell adhesion in all regions at Re = 100 and 140, compared to an uncoated model under the same flow conditions. In regions where the cells near the wall were abundant (taper and stenosis), adhesion to E-selectin correlated with the reciprocal of local wall shear stress when flow was steady. Pulsatile now distributed the adherent cells more evenly throughout the coated model. Hence, characterizing both the local hemodynamics and the biological activity on the vessel wall is important in leukocyte adhesion. (C) 2000 Elsevier Science Ltd. All rights reserved.