Characterization of nitric oxide synthase activity in rabbit uterus and vagina: Downregulation by estrogen
Characterization of nitric oxide synthase activity in rabbit uterus and vagina: Downregulation by estrogen
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DOI：
10.1016/s0024-3205(98)00184-2
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发表时间：
1998-05-01
期刊：
影响因子：
6.1
通讯作者：
Al-Hijji, J
中科院分区：
文献类型：
--
作者：
Batra, S;Al-Hijji, J
用一氧化氮合酶(NOS)测定了兔子宫、阴道和小脑的胞浆和颗粒部分的一氧化氮合酶(NOS)活性，并研究了雌激素对其活性的影响。胞质和颗粒组分中的一氧化氮合酶活性均高度依赖于钙离子，胞质组分的活性比三种器官的颗粒组分的活性高近4倍。一氧化氮合酶含量以小脑最高，其次为阴道和子宫。阴道一氧化氮合酶活性是子宫一氧化氮合酶的3-4倍。连续给药一周后，子宫和阴道胞质一氧化氮合酶分别下降近7倍和L倍，颗粒一氧化氮合酶则无明显变化。雌激素处理对小脑胞质或颗粒一氧化氮合酶无影响。雌激素对子宫、阴道等雌激素靶组织胞质一氧化氮合酶的下调，对小脑无影响，具有重要的生理意义。
Nitric oxide synthase (NOS) was determined in the soluble (cytosolic) and particulate fractions of rabbit uterus, vagina and cerebellum and the influence of estrogen treatment on NOS activity was studied. NOS in both the cytosolic and particulate fractions was highly calcium dependent, The activity in cytosolic fraction was nearly 4-fold higher than the particulate fractions from all three organs. The concentration of NOS was highest in cerebellum followed by vagina and uterus. Vaginal NOS activity was 3-4-fold higher than the uterine NOS. After a continuous treatment of rabbits for one week with estrogen, cytosolic NOS was reduced by nearly 7 and l-fold in the uterus and vagina, respectively, whereas there was no significant change in the particulate NOS. Estrogen treatment caused no change in cytosolic or particulate NOS from the cerebellum. Downregulation of cytosolic NOS by estrogen in the estrogen target tissues like uterus and vagina and absence of effect in the cerebellum strongly suggests a physiological significance.