A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP®, RFLP and SSR markers
A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP®, RFLP and SSR markers
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DOI：
10.1023/a:1014831302392
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发表时间：
2002-03-01
影响因子：
5.1
通讯作者：
Klein, PE
中科院分区：
文献类型：
--
作者：
Menz, MA;Klein, RR;Klein, PE
利用AFLP技术，以高粱BTx 623 × IS 3620 C的重组自交系群体为材料，构建了高粱基因组的高密度遗传图谱。1713 cM的地图包括2926个位点分布在10个连锁群，这些位点中的2454个是从EcoRI/MseI或PstI/MseI酶组合产生的AFLP产物。在非AFLP标记中，136个是先前在高粱中定位的SSR，203个是来自水稻、大麦、燕麦和玉米的cDNA和基因组克隆。后一组标记已经在各种草种中作图，因此，可以作为比较作图中的参考标记。在近3000个标记中，有692个标记的LOD值大于或等于3.0，其余标记的LOD值较低
Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the EcoRI/MseI or PstI/MseI enzyme combinations. Among the non-AFLP markers, 136 are SSRs previously mapped in sorghum, and 203 are cDNA and genomic clones from rice, barley, oat, and maize. This latter group of markers has been mapped in various grass species and, as such, can serve as reference markers in comparative mapping. Of the nearly 3000 markers mapped, 692 comprised a LOD greater than or equal to3.0 framework map on which the remaining markers were placed with lower resolution (LOD