Rapid functionalisation and detection of viruses via a novel Ca 2+ -mediated virus-DNA interaction
Rapid functionalisation and detection of viruses via a novel Ca 2+ -mediated virus-DNA interaction
复制标题
通过新型 Ca 2 介导的病毒-DNA 相互作用快速功能化和检测病毒
DOI：
10.1101/629303
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Robb N
中科院分区：
文献类型：
--
作者：
Robb N
目前的病毒检测方法通常需要很长的时间，或者在敏感性和特异性方面受到限制。近几十年来，病毒暴发的频率和规模越来越大，因此迫切需要简便、敏感、快速和廉价的诊断方法。在这里，我们描述和描述了一种新颖的、钙介导的包膜病毒表面与DNA的相互作用，这种作用可以通过连接到DNA上的化学基团来实现完整病毒颗粒的功能化。使用荧光团修饰的DNA，我们已经展示了使用单颗粒跟踪和颗粒大小测定来快速和灵敏地标记和检测流感和其他病毒。使用这种方法，我们在短短一分钟内就检测到了流感临床分离株，比现有的快速诊断测试快了很多。这项强大的技术很容易扩展到其他广泛的包膜致病病毒，并有望成为未来的诊断工具。
Current virus detection methods often take significant time or can be limited in sensitivity and specificity. The increasing frequency and magnitude of viral outbreaks in recent decades has resulted in an urgent need for diagnostic methods that are facile, sensitive, rapid and inexpensive. Here, we describe and characterise a novel, calcium-mediated interaction of the surface of enveloped viruses with DNA, that can be used for the functionalisation of intact virus particles via chemical groups attached to the DNA. Using DNA modified with fluorophores, we have demonstrated the rapid and sensitive labelling and detection of influenza and other viruses using single-particle tracking and particle-size determination. With this method, we have detected clinical isolates of influenza in just one minute, significantly faster than existing rapid diagnostic tests. This powerful technique is easily extendable to a wide range of other enveloped pathogenic viruses and holds significant promise as a future diagnostic tool.