Analysis of Temporal Changes in Growth and Gene Expression for Commensal Gut Microbes in Response to the Polyphenol Naringenin.
Analysis of Temporal Changes in Growth and Gene Expression for Commensal Gut Microbes in Response to the Polyphenol Naringenin.
复制标题
DOI：
10.1177/1178636118775100
复制
发表时间：
2018
影响因子：
--
通讯作者：
Xiao W
中科院分区：
文献类型：
--
作者：
Firrman J;Liu L;Argoty GA;Zhang L;Tomasula P;Wang M;Pontious S;Kobori M;Xiao W
在本研究中，分析了黄烷酮柚皮素对共生肠道微生物（高氏瘤胃球菌、链状双歧杆菌和可可肠球菌）生长和基因表达的影响。生长曲线分析表明，柚皮素对高氏瘤胃球菌没有影响，柚皮素对链状双歧杆菌有轻微增强作用，柚皮素对肠球菌有严重抑制作用。使用单分子 RNA 测序确定柚皮素引起的基因表达变化。分析揭示了柚皮素的以下反应：高氏瘤胃球菌上调与铁吸收相关的基因；链状双歧杆菌上调涉及细胞代谢、DNA修复和分子运输的基因，下调涉及胸苷生物合成和代谢的基因；肠球菌上调参与转录和蛋白质运输的途径，下调负责糖运输和嘌呤合成的基因。首次记录了共生肠道细菌响应柚皮素而发生的生长和基因表达变化。
In this study, the effect of the flavanone naringenin on the growth and genetic expression of the commensal gut microbes, Ruminococcus gauvreauii, Bifidobacterium catenulatum, and Enterococcus caccae, was analyzed. Analysis of growth curves revealed that Ruminococcus gauvreauii was unaffected by naringenin, Bifidobacterium catenulatum was slightly enhanced by naringenin, and Enterococcus caccae was severely inhibited by naringenin. Changes in genetic expression due to naringenin were determined using single-molecule RNA sequencing. Analysis revealed the following responses to naringenin: Ruminococcus gauvreauii upregulated genes involved in iron uptake; Bifidobacterium catenulatum upregulated genes involved in cellular metabolism, DNA repair and molecular transport, and downregulated genes involved in thymidine biosynthesis and metabolism; Enterococcus caccae upregulated pathways involved in transcription and protein transport and downregulated genes responsible for sugar transport and purine synthesis. For the first time, changes in growth and gene expression for commensal gut bacteria in response to naringenin were documented.