Growth studies of subcutaneous rat tumours: comparison of 31P-NMR spectroscopy, acid extracts and histology.
Growth studies of subcutaneous rat tumours: comparison of 31P-NMR spectroscopy, acid extracts and histology.
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DOI：
10.1038/bjc.1989.343
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发表时间：
1989-11
影响因子：
8.8
通讯作者：
Griffiths, J R
中科院分区：
文献类型：
--
作者：
Stubbs, M;Rodrigues, L M;Griffiths, J R
获得了三种皮下植入的大鼠肿瘤（Morris 肝癌 7777、GH3 催乳素瘤、Walker 癌肉瘤）和 N-甲基-N-亚硝基脲诱导的不同生长阶段的大鼠乳腺癌的 31P-NMP、表面线圈谱，并与 NMR 采集后立即获取的组织学切片进行比较。将根据 NMR 谱计算的代谢物比率（磷酸肌酸 (PCr)/β 核苷三磷酸 (β NTP)、PCr/Pi、β NTP/Pi）与从类似大小的肿瘤的酸提取物获得的比率进行比较。还测量了肌酸和 ADP。通过核磁共振和提取物测量，其中三个肿瘤显示肿瘤大小增加与代谢物比率降低之间呈正相关，而沃克癌肉瘤显示大小与任何测量参数之间没有相关性。在肿瘤覆盖的皮肤提取物中测量的磷代谢物比率表明，当有肿瘤侵入皮肤的组织学证据时，高能磷酸盐会下降。大鼠皮下生长或诱导的肿瘤的表面线圈 31P-NMR 谱代表了随着肿瘤尺寸增大而缓慢变化的稳态。我们得出的结论是，对 NMR 谱的贡献主要是缺氧肿瘤细胞数量的增加，而不是大面积的坏死组织。
31P-NMP, surface coil spectra of three subcutaneously implanted rat tumours (Morris hepatoma 7777, GH3 prolactinoma, Walker carcinosarcoma) and an N-methyl-N-nitrosourea induced rat mammary adenocarcinoma at different stages of growth were obtained and compared with histological sections taken immediately after NMR acquisitions. Metabolite ratios (phosphocreatine (PCr)/beta nucleoside triphosphate (beta NTP), PCr/Pi, beta NTP/Pi) calculated from the NMR spectra were compared with ratios obtained from acid extracts of tumours of similar size. Measurements of creatine and ADP were also made. Three of the tumours showed positive correlations between increasing tumour size and decreasing metabolite ratios measured both by NMR and in extracts, whereas the Walker carcinosarcoma showed no correlation between size and any parameters measured. Phosphorus metabolite ratios, measured in extracts of skin overlying the tumours, indicated a fall in high energy phosphate when there was histological evidence of skin invasion by the tumour. Surface coil 31P-NMR spectra of subcutaneously grown or induced tumours in the rat represent a slowly changing steady state as the tumour increases in size. We conclude that increasing numbers of hypoxic tumour cells, rather than large areas of necrotic tissue, contribute largely to the NMR spectrum.