Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells
Gelatin methacrylate as a promising hydrogel for 3D microscale organization and proliferation of dielectrophoretically patterned cells
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DOI：
10.1039/c2lc40213k
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Matsue, Tomokazu
中科院分区：
文献类型：
--
作者：
Ramon-Azcon, Javier;Ahadian, Samad;Matsue, Tomokazu
建立细胞的3D微尺度组织具有许多实际应用，例如在确定细胞命运（例如，在细胞中）中。例如，在一个实施例中，增殖、迁移、分化和凋亡）和制备功能性组织构建体。空间图案化细胞的一种方法是通过介电泳（DEP）。DEP具有对细胞操作重要的特征，例如高准确性、速度、可扩展性以及处理粘附和非粘附细胞的能力。然而，这种方法的广泛应用在很大程度上受到限制，因为用于细胞包封的合适水凝胶的数量有限。迄今为止，聚乙二醇-二丙烯酸酯（PEG-DA）和琼脂糖已被广泛用于细胞的介电图案化。在这项研究中，我们提出明胶甲基丙烯酸酯（GelMA）作为一种有前途的水凝胶用于细胞介电图案化，因为它的生物相容性和低粘度。与PEG水凝胶相比，GelMA水凝胶在制作成肌细胞（C2 C12）和内皮细胞（HUVEC）的细胞图案以及维持细胞活力和生长方面表现出上级性能。我们还开发了一种简单而强大的协议，这些细胞的共培养。GelMA水凝胶和DEP技术的组合应用适用于以快速、准确和可扩展的方式创建高度复杂的微尺度组织，在基础细胞生物学和再生医学中具有重要应用。
Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e. g., proliferation, migration, differentiation, and apoptosis) and in making functional tissue constructs. One approach to spatially pattern cells is by dielectrophoresis (DEP). DEP has characteristics that are important for cell manipulation, such as high accuracy, speed, scalability, and the ability to handle both adherent and non-adherent cells. However, widespread application of this method is largely restricted because there is a limited number of suitable hydrogels for cell encapsulation. To date, polyethylene glycol-diacrylate (PEG-DA) and agarose have been used extensively for dielectric patterning of cells. In this study, we propose gelatin methacrylate (GelMA) as a promising hydrogel for use in cell dielectropatterning because of its biocompatibility and low viscosity. Compared to PEG hydrogels, GelMA hydrogels showed superior performance when making cell patterns for myoblast (C2C12) and endothelial (HUVEC) cells as well as in maintaining cell viability and growth. We also developed a simple and robust protocol for co-culture of these cells. Combined application of the GelMA hydrogels and the DEP technique is suitable for creating highly complex microscale tissues with important applications in fundamental cell biology and regenerative medicine in a rapid, accurate, and scalable manner.