Association of serum interleukin-33 level and the interleukin-33 genetic variant with Japanese cedar pollinosis
Association of serum interleukin-33 level and the interleukin-33 genetic variant with Japanese cedar pollinosis
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DOI：
10.1111/j.1365-2222.2008.03114.x
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发表时间：
2008-12-01
影响因子：
6.1
通讯作者：
Tamari, M.
中科院分区：
文献类型：
--
作者：
Sakashita, M.;Yoshimoto, T.;Tamari, M.
IL-33是一种IL-1样细胞因子，是IL-1 RL 1的配体，IL-1 RL 1是2型T辅助反应的重要效应分子。虽然IL-33/IL-1 RL 1相互作用在诱导过敏性气道炎症中起重要作用，但血清IL-33水平和IL-33基因多态性在人类过敏性疾病中的遗传影响尚不清楚，本研究的目的是检测血清IL-33水平和IL-33基因多态性是否与日本雪松花粉症相关，我们使用HapMap数据库对该基因进行了连锁不平衡（LD）作图，并对两个选择的标签单核苷酸多态性进行了基因分型。我们对JC花粉症患者（n=170）和正常对照组（n = 100）进行了IL-33的相关性研究，用ELISA法检测了270例JC花粉症患者血清IL-33水平，JC花粉症患者血清IL-33水平显著高于正常对照组（P=0.0018）。在遗传关联分析中，我们发现该多态性与JC花粉症呈正相关（P=0.048），我们的结果支持IL-33在JC花粉症的发病机制中的作用。Sakashita，T. Yoshimoto，T. Hirota，M. Harada，K. Okubo，Y. Osawa，S.藤田，Y。中村，K. Yasuda，K. Nakanishi和M. Tamari，临床和实验过敏，2008（38）1875-1881。
IL-33, an IL-1-like cytokine, is a ligand for IL1RL1, which is an important effector molecule of type 2 T helper responses. Although IL-33/IL1RL1 interaction has been suggested to be important in induction of allergic airway inflammation, serum levels of IL-33 and the genetic influences of the polymorphisms of IL-33 in human allergic diseases are unclear.The aim of this study was to examine whether the serum IL-33 level and polymorphisms in IL-33 are associated with Japanese cedar (JC) pollinosis, the most common form of allergic rhinitis, and a major public health problem, in Japan.We performed linkage disequilibrium (LD) mapping of the gene using the HapMap database, and two selected tag single nucleotide polymorphisms were genotyped. We conducted an association study of IL-33 (JC pollinosis, n=170; normal controls, n=100) and measured the IL-33 levels in sera of the 270 subjects by ELISA.Serum levels of IL-33 were significantly higher in patients with JC pollinosis (P=0.0018) than in controls. In genetic association analysis, we found a positive association between the polymorphism and JC pollinosis (P=0.048).Our results support a role for IL-33 in the pathogenesis of JC pollinosis.Cite this as: M. Sakashita, T. Yoshimoto, T. Hirota, M. Harada, K. Okubo, Y. Osawa, S. Fujieda, Y. Nakamura, K. Yasuda, K. Nakanishi and M. Tamari, Clinical and Experimental Allergy, 2008 (38) 1875-1881.