Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer
Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer
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鉴定神经胶质成熟因子β作为浆液性卵巢癌的独立预后预测因子
DOI：
10.1016/j.ejca.2010.04.015
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发表时间：
2010-07-01
影响因子：
8.4
通讯作者：
Xie, Xing
中科院分区：
文献类型：
--
作者：
Li, Yan Li;Ye, Feng;Xie, Xing
浆液性卵巢癌（SOC）是上皮性卵巢癌最常见的亚型，仍然是妇科恶性肿瘤死亡的主要原因。对浆液性卵巢癌相关蛋白的进一步了解可能会带来新的治疗靶点、早期检测或预后预测的新标志物。在这项研究中，我们应用蛋白质组学技术分析了SOC和正常卵巢上皮组织的蛋白质表达谱。2-DE联合MALDI-TOF/TOF共鉴定出54个异常表达蛋白。其中6个蛋白经western blot验证。采用实时定量逆转录聚合酶链反应（RT-PCR）对这些蛋白进行相应的基因表达分析。此外，我们通过免疫组织化学分析了246例不同程度卵巢上皮病变患者的神经胶质成熟因子β (GMFB)蛋白表达。GMFB在SOC中的表达明显高于正常上皮、良性浆液腺瘤和交界性浆液腺瘤组织，且与FIGO分期呈正相关（P = 0.012）。GMFB高表达与不良的无病生存期（P = 0.010）和总生存期（P = 0.003）相关，而多因素分析显示GMFB是SOC患者无病生存期（P = 0.026）和总生存期（P = 0.006）的独立预后因素。因此，我们认为这里鉴定的蛋白质可能参与了SOC的发生或进展，GMFB可以被认为是SOC患者的预后预测因子。(C) 2010 Elsevier Ltd.版权所有。
Serous ovarian carcinoma (SOC) is the most common subtype of epithelial ovarian cancer which remains the leading cause of death from gynaecologic malignancy. Further knowledge of the proteins involved in serous ovarian cancer may lead to new treatment targets, new markers for early detection or prognosis prediction. In this study, we applied proteomic techniques to analyse the protein expression profiles of SOC and normal ovarian epithelium tissues. Totally 54 aberrantly expressed proteins were identified using 2-DE combined with MALDI-TOF/TOF. Six of these proteins were validated by western blot. Corresponding gene expression analysis of these proteins was also performed using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Additionally, we analysed glia maturation factor beta (GMFB) protein expression by immunohistochemistry in 246 patients with various degrees of ovarian epithelial lesions. GMFB expression in SOC was found to be significantly enhanced than that in normal epithelium, benign serous adenoma and borderline serous adenoma tissues, and was positively correlated with FIGO stage (P = 0.012). High GMFB expression was associated with poor disease-free survival (P = 0.010) and overall survival (P = 0.003), while multivariate analysis revealed GMFB to be an independent prognostic factor for disease-free survival (P = 0.026) and overall survival (P = 0.006) in patients with SOC. We therefore propose that proteins identified here may be involved in the development or progression of SOC, and GMFB can be considered as a prognostic predictor for SOC patients. (C) 2010 Elsevier Ltd. All rights reserved.