The relationship between the occurrence of intractable epilepsy with glial cells and myelin sheath an experimental study
The relationship between the occurrence of intractable epilepsy with glial cells and myelin sheath an experimental study
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顽固性癫痫的发生与胶质细胞和髓鞘关系的实验研究
DOI：
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发表时间：
2016
影响因子：
3.3
通讯作者：
Yuan p
中科院分区：
文献类型：
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作者：
Hu X;Wang JY;Gu R;Qu H;Li M;Chen L;Liu R;Yuan p
目的：癫痫的发生与髓鞘损伤有关，少突胶质细胞（oligodendrocyte，OL）是髓鞘的主要细胞，本研究观察了难治性癫痫（intensive epilepsy，IE）患者不同阶段的癫痫病灶处OL、脱髓鞘和髓鞘相关蛋白的变化，为IE的病理生理学研究提供有用的信息。患者和方法：收集行致痫灶切除术的IE患者的病历资料，并进行术后随访。取10例非脑功能障碍患者的脑组织作为对照。免疫荧光双染法观察OL的表达。用勒克索坚牢蓝（LFB）染色法观察癫痫灶脱髓鞘情况。采用实时荧光定量PCR、Western blot方法检测髓鞘相关蛋白的表达。结果：实验组前体细胞、前体细胞增多，成熟细胞减少，与对照组比较，P < 0.05。实验组脱髓鞘率明显高于对照组（P < 0.01）。癫痫灶内髓鞘碱性蛋白（MBP）、少突胶质细胞髓鞘糖蛋白（OMgp）、髓鞘相关糖蛋白（MAG）及其他MAG相关蛋白-Nogo受体（NgRs）和GD 1 α的表达均显著降低（p < 0.01）。结论：IE患者癫痫灶内OL分化异常，脱髓鞘、脱髓鞘及髓鞘相关蛋白减少与IE的发生有关。
OBJECTIVE: The occurrence of epilepsy is associated with myelin sheath injury; oligodendrocyte (OL) is the main cell of myelin sheath; In this study, we observed the changes of OL, demyelination, and myelin associated protein in different stages of intractable epilepsy (IE) at the epileptic foci of patients, and provide useful information for the pathophysiology of IE. PATIENTS AND METHODS: IE patients who received epileptogenic focus resection were recruited as the experimental group, their medical records were collected and postoperative follow up was performed. The brain tissues from10 cases with non-brain disorders were obtained as controls. Immunofluorescence double staining was used to observe OL expression. The demyelination in epileptic foci was observed by luxol fast blue (LFB) staining method. Real-time fluorescent quantitative (RT) PCR, Western blot methods were used to detect the expressions of myelin-related proteins. RESULTS: We observed increased OL precursor cells, former OL and decreased mature OL in experimental group when compared with controls both p < 0.05). The demyelination was obviously higher in experimental group when compared with controls (p < 0.01). We also observed significantly decreasing of myelin basic protein (MBP), oligodendrocytes myelin glycoprotein (OMgp), myelin associated glycoprotein (MAG) and other MAG associated proteins-Nogo receptors (NgRs) and GD1α (p < 0.01) in epileptic foci. CONCLUSIONS: OL is differentiated abnormally at epileptic foci of patients with IE; the demyelination, decreasing of demyelination and myelin associated protein are related to the occurrence of IE.