Innate immunity:: Eggs of Manduca sexta are able to respond to parasitism by Trichogramma evanescens
Innate immunity:: Eggs of Manduca sexta are able to respond to parasitism by Trichogramma evanescens
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DOI：
10.1016/j.ibmb.2007.10.001
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发表时间：
2008-02-01
影响因子：
3.8
通讯作者：
Hilker, M.
中科院分区：
文献类型：
--
作者：
Abdel-latief, M.;Hilker, M.
迄今为止，尚不清楚昆虫卵的免疫反应是通过寄生来诱导还是抑制。我们研究了天蛾卵中免疫相关基因的转录是否因赤眼蜂的寄生而发生变化。首先，使用 DDRT-PCR，从鸡蛋中分离出几种已知代表可通过细菌攻击诱导的免疫相关 M. sexta 基因的 cDNA 元件。此外，还发现了两种新的 cDNA：(a) immulectin-V（IML-V 被认为参与异物识别，以及 (b) 一种新的类似 Moricin 蛋白，可能具有抗菌作用 (L-Mor)。定量实时 RT-PCR 分析显示，与未寄生鸡蛋相比，被寄生鸡蛋中 IML-V、原酚氧化酶 (ProPO)、原酚氧化酶激活蛋白酶 I (PAP 1) 和原酚氧化酶激活蛋白酶 I (PAP 1) 的转录增强。寄生虫卵和未寄生虫卵之间未检测到编码抗菌肽 L-Mor、亮肽素 Leu 和 attacin II Att II 的序列。寄生 3 天（天蚕素 6、Cec 6）和 2 天（gloverin、Glov）后，其他抗菌肽的转录水平均受到抑制。在接触寄生蜂后，仅 64% 的赤眼蜂样本中含有寄生蜂幼虫。我们的数据表明，天蛾卵的免疫系统对寄生表现出不同的反应，并表明昆虫卵可以抵御寄生。 (C) 2007 Elsevier Ltd. 保留所有权利。
So far, it was unknown whether immune responses of insect eggs are inducible or suppressed by parasitism. We investigated whether transcription of immune related genes in eggs of Manduca sexta changed in response to parasitism by Trichogramma evanescens. First, using DDRT-PCR, several cDNA elements known to represent immune related M. sexta genes inducible by bacterial challenge were isolated from eggs. In addition, two novel cDNAs were found: (a) immulectin-V (IML-V suggested to be involved in recognition of foreign bodies, and (b) a new like-moricin protein with possible antimicrobial effects (L-Mor). Quantitative real time RT-PCR analyses revealed enhanced transcription in parasitized eggs compared to unparasitized ones for IML-V, prophenoloxidase (ProPO), prophenoloxidase activating protease I (PAP 1), and proparalytic peptide (ProPP). No significant differences between parasitized and unparasitized eggs were detected for sequences encoding the antimicrobial peptides L-Mor, leureptin Leu, and attacin II Att II. Transcript levels of other antibacterial peptides were suppressed after parasitization for 3 d (cecropin 6, Cec 6) and 2 d (gloverin, Glov). While nearly 100% of the Manduca eggs contained Trichogramma specimens I d after exposure to parasitoids, only 64% of the host eggs harbored parasitoid larvae 4 d after parasitization. Our data demonstrate that the immune system of Manduca eggs shows differentiated responses to parasitization and suggest that insect eggs can defend against parasitization. (C) 2007 Elsevier Ltd. All rights reserved.