CACO-2 CELL-LINE - A SYSTEM FOR STUDYING INTESTINAL IRON TRANSPORT ACROSS EPITHELIAL-CELL MONOLAYERS
CACO-2 CELL-LINE - A SYSTEM FOR STUDYING INTESTINAL IRON TRANSPORT ACROSS EPITHELIAL-CELL MONOLAYERS
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DOI：
10.1016/0005-2736(91)90165-5
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发表时间：
1991-11-18
期刊：
影响因子：
--
通讯作者：
GLASS, J
中科院分区：
文献类型：
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作者：
ALVAREZHERNANDEZ, X;NICHOLS, GM;GLASS, J
利用生长在双室培养的Caco-2细胞研究了铁跨极化肠上皮的转运。 当细胞在低、正常或高铁浓度条件下生长时，不仅细胞的铁含量显著改变，而且低铁细胞的跨上皮电阻（TEER）增加近2倍。 Fe-59从顶面进入细胞和运输到基底室的吸收受到铁的价态和细胞的铁状态的影响。 从Fe-59（II）-抗坏血酸盐的摄取约为600 pmol Fe-59/h/mg蛋白质，在低铁细胞中增加约2倍，并且比从螯合到次氮基三乙酸、BSA或柠檬酸盐的Fe-59（III）的摄取高约13-200倍。 从Fe-59（II）-抗坏血酸盐转运至基底室的速率分别为3.7 +/- 1.7 pmol/h/cm 2（对于Fe缺乏细胞）和0.72 +/- 0.1 pmol/h/cm 2（对于正常铁细胞），从Fe-59（III）-BSA转运至基底室的速率分别为1.1 +/- 0.2 pmol/h/cm 2（对于Fe缺乏细胞）和0.3 +/- 0.03 pmol/h/cm 2（对于正常铁细胞）。 从Fe（II）和在缺铁细胞中的铁的更大转运支持使用Caco-2细胞作为铁转运的模型。
Iron transport across polarized intestinal epithelium was studied by using Caco-2 cells grown in bicameral chambers. When cells were grown under conditions of low, normal, or high iron concentration not only was the iron content of the cells markedly altered but the low iron cells exhibited a nearly 2-fold increase in transepithelial electrical resistance (TEER). Fe-59 uptake from the apical surface into cells and transport into the basal chamber was affected both by the valency of the iron and the iron status of the cells. Uptake from Fe-59(II)-ascorbate was about 600 pmol Fe-59/h per mg protein, increased about 2-fold in low iron cells, and was about 13-200-fold greater than uptakes from Fe-59(III) chelated to nitrilotriacetic acid, BSA, or citrate. Transport into the basal chamber from Fe-59(II)-ascorbate was 3.7 +/- 1.7 pmol/h per cm2 for Fe-deficient cells vs. 0.72 +/- 0.1 pmol/h per cm2 for normal-Fe cells and from Fe-59(III)-BSA 1.1 +/- 0.2 pmol/h per cm2 vs. 0.3 +/- 0.03 pmol/h per cm2 for deficient vs. normal iron cells, respectively. The greater transport of iron both from Fe(II) and in iron deficient cells supports the use of the Caco-2 cells as a model for iron transport.